Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair in a goat model

Journal of Tissue Engineering and Regenerative Medicine
Dekai XiaWei Fu

Abstract

Traditional treatment therapies for tracheal stenosis often cause severe post-operative complications. To solve the current difficulties, novel and more suitable long-term treatments are needed. A whole-segment tissue-engineered trachea (TET) representing the native goat trachea was 3D printed using a poly(caprolactone) (PCL) scaffold engineered with autologous auricular cartilage cells. The TET underwent mechanical analysis followed by in vivo implantations in order to evaluate the clinical feasibility and potential. The 3D-printed scaffolds were successfully cellularized, as observed by scanning electron microscopy. Mechanical force compression studies revealed that both PCL scaffolds and TETs have a more robust compressive strength than does the native trachea. In vivo implantation of TETs in the experimental group resulted in significantly higher mean post-operative survival times, 65.00 ± 24.01 days (n = 5), when compared with the control group, which received autologous trachea grafts, 17.60 ± 3.51 days (n = 5). Although tracheal narrowing was confirmed by bronchoscopy and computed tomography examination in the experimental group, tissue necrosis was only observed in the control group. Furthermore, an encouraging epitheli...Continue Reading

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Citations

Feb 18, 2020·Journal of Tissue Engineering and Regenerative Medicine·Archna DhasmanaSagar Rawal
Aug 31, 2020·Laryngoscope Investigative Otolaryngology·Weston L NiermeyerTendy Chiang
Nov 4, 2020·Tissue Engineering. Part C, Methods·Jakob M TownsendMichael S Detamore
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Alexander J BoysRoisin M Owens
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Yunlang SheChang Chen
Jun 5, 2021·Tissue Engineering. Part B, Reviews·Julie Lien Paternoster, Jan Jeroen Vranckx

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