Tissue engineering approach to repair abdominal wall defects using cell-seeded bovine tunica vaginalis in a rabbit model.

Journal of Materials Science. Materials in Medicine
T AyeleMohamed M Noordin

Abstract

The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3 x 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of the first group were repaired with myoblast-seeded-bovine tunica vaginalis whereas the second group repaired with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at 7th, 14th, and 30th days of post-implantation from each group and the explanted specimens were subjected to macroscopic and microscopic analysis. In every case, seeded scaffolds have better deposition of newly formed collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibers were only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine tunica vaginalis can be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal muscle tissue.

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Citations

Dec 23, 2011·Artificial Organs·Lu ZhangYan Gu
Nov 8, 2015·Hernia : the Journal of Hernias and Abdominal Wall Surgery·A KlingerT N Tulenko
Feb 9, 2017·Tissue Engineering. Part a·J A PassipieriGeorge J Christ
Mar 19, 2019·Tissue Engineering. Part a·Juliana A PassipieriGeorge J Christ
Oct 8, 2014·Plastic and Reconstructive Surgery. Global Open·Justin M BroylesCharles E Butler
Jun 26, 2015·Advanced Healthcare Materials·Olivier GuillaumeAlexander Petter-Puchner
Feb 26, 2020·Hernia : the Journal of Hernias and Abdominal Wall Surgery·O GuillaumeR Fortelny
Mar 21, 2020·Tissue Engineering. Part B, Reviews·Jack WhooleyNiall F Davis

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