Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Wanyao XiaY Cao

Abstract

Chitosan has been shown to be a promising scaffold for various applications in tissue engineering. In this study, a chitosan-gelatin complex was fabricated as a scaffold by a freezing and lyophilizing technique. Chitosan's structure and characteristics are similar to those of glycosaminoglycan (GAG) and its analogs, and possesses various biological activities, whereas gelatin can serve as a substrate for cell adhesion, differentiation, and proliferation. With the use of autologous chondrocytes isolated from pig's auricular cartilage and seeded onto the chitosan-gelatin scaffold, elastic cartilages have been successfully engineered at the porcine abdomen subcutaneous tissue. After 16 weeks of implantation, the engineered elastic cartilages have acquired not only normal histological and biochemical, but also mechanical properties. The tissue sections of the engineered elastic cartilages showed that the chondrocytes were enclosed in the lacuna, similar to that of native cartilage. The presence of elastic fibers in the engineered cartilages was also demonstrated by Vehoeff's staining, and immunohistochemical staining confirmed the presence of type II collagen in the engineered cartilages. Quantitatively, the GAG in the engineered c...Continue Reading

References

Oct 1, 1993·The Journal of Veterinary Medical Science·Y OkamotoS Tokura
Jul 9, 1999·Medical & Biological Engineering & Computing·M Mattioli-BelmonteF Greco
Dec 22, 1999·Journal of Biomedical Materials Research·V F SechriestJ K Suh
Apr 25, 2000·Journal of Periodontology·Y M LeeC P Chung
Jul 6, 2000·Journal of Biomedical Materials Research·A LahijiC G Frondoza
Jan 5, 2002·Journal of Biomedical Materials Research·Pamela J VandeVordPaul H Wooley

❮ Previous
Next ❯

Citations

Jul 18, 2012·Bioprocess and Biosystems Engineering·Cristian A AcevedoJavier Enrione
May 28, 2013·Bioprocess and Biosystems Engineering·Javier EnrioneCristian A Acevedo
Jun 19, 2008·Journal of Materials Science. Materials in Medicine·Neethu MohanYasuhiko Tabata
May 17, 2005·Biomaterials·Alberto Di MartinoMakarand V Risbud
Sep 14, 2006·Tissue Engineering·Suzy DufloGlenn D Prestwich
Aug 29, 2009·Tissue Engineering. Part a·Sriram RavindranAnne George
Aug 29, 2013·Tissue Engineering. Part a·David A BicharaCathryn A Sundback
Mar 8, 2008·Journal of Biomaterials Science. Polymer Edition·Lin LiuJuming Yao
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Melika SaremV Prasad Shastri
Jun 12, 2008·Journal of Materials Science. Materials in Medicine·Kris GellynckGust Verbruggen
Jan 10, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Qiong WuGuo-Qiang Chen
Nov 8, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Maryam HajiabbasFarzaneh Sharifiaghdas
Feb 18, 2016·International Journal of Biomaterials·Kanchan MajiAkalabya Bissoyi
Nov 22, 2011·The Journal of Surgical Research·Jiang JiangShuzhong Guo
Apr 4, 2009·Journal of Structural Biology·Julie A LastChristopher J Murphy
Jan 6, 2009·Acta Biomaterialia·W W Thein-Han, R D K Misra
Nov 3, 2007·Advanced Drug Delivery Reviews·Cindy Chung, Jason A Burdick
May 29, 2007·International Journal of Pediatric Otorhinolaryngology·B H I RuszymahB S Aminuddin
Feb 29, 2008·Biotechnology and Bioengineering·J V NygaardF Besenbacher
Sep 30, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Junjie LiKangde Yao
Apr 5, 2012·The Journal of Pharmacy and Pharmacology·Roberta CassanoNevio Picci
Jun 10, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hailin ZhuJianyong Chen
Mar 29, 2011·Journal of Biomedical Materials Research. Part a·Kai WeiIck-Soo Kim
Aug 10, 2013·The Journal of Pharmacy and Pharmacology·Maria L MancaMaria Manconi
May 23, 2012·Journal of Biomedical Materials Research. Part a·Piergiorgio GentileGianluca Ciardelli
Dec 12, 2012·Journal of Tissue Engineering and Regenerative Medicine·G KhangR L Reis
Jul 12, 2005·Biomaterials·Yan HuangSundararajan V Madihally
Aug 11, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Aditya AroraDhirendra S Katti
Aug 8, 2015·Annual Review of Chemical and Biomolecular Engineering·A M Ross, J Lahann
Nov 6, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Chia-Ling KoWen-Cheng Chen
Feb 18, 2014·Acta Biomaterialia·Andreas H TeuschlHeinz Redl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.