Tissue Engineering of Necrotic Dental Pulp of Immature Teeth with Apical Periodontitis in Dogs: Radiographic and Histological Evaluation

The Journal of Clinical Pediatric Dentistry
Eman A El AshiryHazem M Atta

Abstract

To evaluate tissue engineering technology to regenerate pulp-dentin like tissues in pulp canals of immature necrotic permanent teeth with apical periodontitis in dogs. The study was performed on 36 teeth in 12 dogs. The experiment was carried out using split mouth design. In each dog 3 teeth were selected for implementing the study procedure. Apical periodontitis was induced in Group A and B teeth. Group (A): immature upper left 2nd permanent incisors that were transplanted with a construct of autologous dental pulp stem cells with growth factors seeded in a chitosn hydrogel scaffold. Group (B): immature upper right 2nd permanent incisor that received only growth factors with scaffold. A third tooth in each dog was selected randomly for isolation of dental pulp stem cells (DPSCs). Both groups were closed with a double coronal seal of white MTA (Mineral trioxide aggregate) and glass ionomer cement. Both groups were monitored radiographically for 4 months and histologically after sacrificing the animals. There was no statistically significant difference in radiographic findings between group (A) and group (B) for healing of radiolucencies, while there was statistically significant difference between group (A) and group (B) regard...Continue Reading

References

Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Nov 22, 2002·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Valentina GiulianiGabriella Pagavino
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
Apr 16, 2004·Journal of Endodontics·Francisco Banchs, Martin Trope
May 5, 2005·Tissue Engineering·W ZhangJ A Jansen
Jul 19, 2006·Tissue Engineering·Jason W CookeWilliam V Giannobile
Dec 22, 2006·PloS One·Wataru SonoyamaSongtao Shi
May 29, 2007·Cytokine & Growth Factor Reviews·Alessandra MiceraFrancesca Levi-Schaffer
Jul 19, 2008·Journal of Endodontics·Mabel M CordeiroJacques E Nör
Aug 1, 2008·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Rania M El-BacklyMona K Marei
Jan 24, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Napoleone Ferrara
Jun 25, 2009·International Endodontic Journal·G T-J Huang
Mar 31, 2010·Journal of Dental Research·L CasagrandeJ E Nör
Apr 17, 2010·Journal of Dental Research·V T SakaiJ E Nör
May 22, 2010·Tissue Engineering. Part a·Jin Y KimJeremy J Mao
Oct 19, 2010·Journal of Endodontics·Flavio F DemarcoJacques E Nör
Jan 29, 2011·Odontology·Luciano CasagrandeJacques E Nör
Feb 19, 2011·Journal of Endodontics·Nozomu YamauchiMitsuo Yamauchi
Jun 17, 2011·Advances in Dental Research·M Nakashima, K Iohara
Jul 19, 2011·Journal of Endodontics·Waruna Lakmal DissanayakaLijian Jin
Jun 5, 2012·Journal of Dental Research·Y ZhengS L Wang
Nov 14, 2012·Journal of Endodontics·Xiaofei ZhuWenhao Zhu
Oct 28, 2015·BioMed Research International·Martin Rodríguez-VázquezLuis Fernando Quiñones-Olvera

❮ Previous
Next ❯

Citations

Jun 25, 2019·International Endodontic Journal·K M Fawzy El-SayedF Schwendicke
Mar 19, 2020·Stem Cells International·Geraldine M AhmedKarim Fawzy El-Sayed
Oct 18, 2019·Frontiers in Pharmacology·Christina M A P SchuhSebastian Aguayo
Dec 16, 2020·Polymers·Marwa M S AbbassKarim M Fawzy El-Sayed
Apr 23, 2021·Tissue Engineering. Part B, Reviews·Francesca MangioneSibylle Vital
May 1, 2021·International Journal of Molecular Sciences·Sahng G Kim
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Dixin CuiMian Wan
Aug 29, 2021·Pharmaceuticals·Gustavo MoraesWalter L Siqueira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.