Tissue evacuated during joint replacement procedure as a source of mononuclear cells

European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie
Jakobsons EriksErglis Andrejs

Abstract

Different cell populations from bone marrow were used in various clinical trials for cardiac diseases during last decade. Four clinical studies are ongoing in our institution and enroll patients with cardiac diseases, coronary disease, type 2 diabetes, and osteoarthritis. The density gradient is used to separate bone marrow mononuclear cells. Joint replacement procedures were associated with significant loss of tissue. Usually, excess tissue as bone marrow, peripheral blood and fat are removed to clean operation site. The aim of this study is to prove whether removed tissue during joint replacement procedure can be considered as a significant source of mononuclear cells. Excised tissue obtained during joint replacement procedure was collected by AutoLog system. Bone marrow tissue was collected by iliac crest puncture. Mononuclear cells from both sources were isolated by using Ficoll density gradient centrifugation. Flow cytometry was used to detect mononuclear cell, CD34+ population counts and cell viability. Tissue processing yields between the group of joint replacement and iliac crest puncture group were compared. Together, 34 bone marrow tissue processings were performed. On average, samples contained 46.31 ± 9.35 ml of bon...Continue Reading

References

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Sep 23, 2006·The New England Journal of Medicine·Volker SchächingerUNKNOWN REPAIR-AMI Investigators
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Methods Mentioned

BETA
flow cytometry

Software Mentioned

ISHAGE
AutoLog

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