Tissue factor expression during coculture of endothelial cells and monocytes

Experimental and Molecular Pathology
J C LewisC J Kirkpatrick

Abstract

The role of monocytes as initiators of coagulation through the expression of tissue factor has been well documented in vitro, and the relationship of monocyte tissue factor to the thrombotic complications of atherosclerosis has been suggested. Tissue factor antigen has been identified in the plasma membranes of monocytes adherent to the vascular endothelium overlying atherosclerotic plaques and the presence of tissue factor in adherent mononuclear cells correlates with the polymerization of fibrin at these same sites. To further understand the relationship of cellular adhesion to tissue factor expression, human monocytes were cocultured for periods ranging from 30 min to 24 hr with endothelial cells isolated from human umbilical veins (HUVEC). Tissue factor antigen, as assayed by both ELISA and immunogold electron microscopy, was minimal on either monocytes or HUVEC maintained in homogeneous cultures or on the cells when cocultured for 1 hr or less. This was true whether the HUVEC were in a native state or if they had been stimulated with interleukin-1 (IL-1 beta) or lipopolysaccharide (LPS) prior to monocyte adhesion. Typically, less than 13% of the cells in short-term cocultures were positively labeled through anti-tissue fac...Continue Reading

Citations

Dec 28, 2006·Annals of Biomedical Engineering·Charles Stevenson WallaceGeorge A Truskey
Jul 29, 2005·Artificial Organs·Pavlina ZemanovaFrantisek Sefrna
Feb 13, 2001·The Journal of Pathology·B A LwaleedA J Cooper
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Salvador GomezZhiwei Hu

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