PMID: 9653181Jul 8, 1998Paper

Tissue factor transcription driven by Egr-1 is a critical mechanism of murine pulmonary fibrin deposition in hypoxia

Proceedings of the National Academy of Sciences of the United States of America
S F YanDavid M Stern

Abstract

Local hypoxemia and stasis trigger thrombosis. We have demonstrated previously that in a murine model of normobaric hypoxia pulmonary fibrin deposition is a result of expression of tissue factor, especially in oxygen-deprived mononuclear phagocytes (MPs). We now show that transcription factor early-growth-response gene product (Egr-1) is rapidly activated in hypoxia, both in vitro and in vivo, and is responsible for transcription and expression of tissue factor in hypoxic lung. MPs and HeLa cells subjected to hypoxia (pO2 approximately 13 torr) had increased levels of tissue factor transcripts (approximately 18-fold) and an increased rate of transcription (approximately 15-fold), based on nuclear run-on analysis. Gel-shift analysis of nuclear extracts from hypoxic MPs and HeLa cells demonstrated increased DNA-binding activity at the serum response region (SRR; -111/+14 bp) of the tissue factor promoter at Egr-1 motifs. Using 32P-labeled Egr consensus oligonucleotide, we observed induction of DNA-binding activity in nuclear extracts from hypoxic lung and HeLa cells because of activation of Egr-1, by means of supershift analysis. Transient transfection of HeLa cells with chimeric plasmids containing wild-type or mutant SRR from t...Continue Reading

References

Sep 1, 1977·Medical Hypotheses·P C Malone
Nov 1, 1978·The Journal of Pathology·T J Chambers
Mar 1, 1981·The British Journal of Surgery·J D HamerI A Silver
Apr 28, 1995·The Journal of Biological Chemistry·S L LeeJ Milbrandt
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·A Gashler, V P Sukhatme
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T MoritaS Kourembanas
Dec 15, 1994·The New England Journal of Medicine·W H GeertsJ P Szalai
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D FirthP J Ratcliffe
Apr 1, 1994·The Journal of Clinical Investigation·M KarakurumD Stern
Feb 14, 1997·The Journal of Biological Chemistry·S F YanD Stern
Feb 1, 1997·Kidney International·P J RatcliffeS M Wood
Apr 4, 1997·Cell·K Guillemin, M A Krasnow
Apr 1, 1997·The Journal of Clinical Investigation·C A LawsonD J Pinsky

❮ Previous
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Citations

Sep 19, 2002·Molecular Reproduction and Development·Phillip J WilderAngie Rizzino
Nov 28, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Rubin M TuderIwona Fijalkowska
Jul 12, 2005·Pflügers Archiv : European journal of physiology·Eoin P Cummins, Cormac T Taylor
Mar 20, 2010·Journal of Bone and Mineral Metabolism·Andrew J LegerNelson S Yew
Aug 25, 2012·Journal of Molecular Histology·Yan-Yan FanXing-Biao Li
Apr 10, 2008·Sleep & Breathing = Schlaf & Atmung·Ali A El SolhKristie R Szarpa
Mar 20, 2003·Current Diabetes Reports·Paresh Dandona
Sep 23, 2003·Mechanisms of Ageing and Development·Hyungsoo KimHong-Duk Youn
Feb 16, 2000·Thrombosis Research·U R Pendurthi, L V Rao
Apr 17, 2003·Biochimica Et Biophysica Acta·Krista K GravenEd Amberger
Oct 26, 2002·Free Radical Biology & Medicine·Daniela Salvemini, Salvatore Cuzzocrea
Mar 13, 2003·Kidney International·Marina CarlHarald D Rupprecht
Mar 30, 2001·The Journal of Biological Chemistry·Z DongJ Nishiyama
Oct 25, 2000·Human Gene Therapy·M BryantM Braddock
May 15, 2002·Antioxidants & Redox Signaling·Stella Kourembanas
Jul 11, 2001·Critical Care Medicine·H ten CateP H Reitsma
Aug 13, 2008·Current Opinion in Hematology·Janusz RakJoanne Yu
Nov 20, 2002·Annals of the New York Academy of Sciences·Marian J EvingerArthur S Tischler
Dec 28, 1999·Annual Review of Cell and Developmental Biology·G L Semenza
Dec 31, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiao-Ru YangJames S K Sham
Jul 5, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eva Nozik-GrayckKurt R Stenmark
Apr 8, 2006·American Journal of Respiratory Cell and Molecular Biology·Paul R ReynoldsJohn R Hoidal
Feb 4, 2009·American Journal of Respiratory Cell and Molecular Biology·Elizabeth L KramerTimothy D Le Cras
Aug 4, 2009·American Journal of Respiratory Cell and Molecular Biology·Thomas A WhiteRobert D Simari
Sep 21, 2000·The Journal of Clinical Investigation·N F Voelkel, R M Tuder
Mar 11, 2000·The Journal of Clinical Investigation·T A McCaffreyT Collins
Mar 11, 2000·The Journal of Clinical Investigation·S F YanD M Stern
Jul 14, 2012·PloS One·Estella Sanchez-GuerreroLevon M Khachigian
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Frank R SharpMyriam Bernaudin
May 1, 2001·Biochemical and Biophysical Research Communications·P HoustonM Braddock
Mar 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Snigdha MishraDavid J Pinsky
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Barbara UzonyiAndreas J R Habenicht
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·K AbeF R Rickles
May 29, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·J L SchwachtgenM Braddock

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