Tissue imaging depth limit of stimulated Raman scattering microscopy

Biomedical Optics Express
Andrew H HillDan Fu

Abstract

Stimulated Raman scattering (SRS) microscopy is a promising technique for studying tissue structure, physiology, and function. Similar to other nonlinear optical imaging techniques, SRS is severely limited in imaging depth due to the turbidity and heterogeneity of tissue, regardless of whether imaging in the transmissive or epi mode. While this challenge is well known, important imaging parameters (namely maximum imaging depth and imaging signal to noise ratio) have rarely been reported in the literature. It is also important to compare epi mode and transmissive mode imaging to determine the best geometry for many tissue imaging applications. In this manuscript we report the achievable signal sizes and imaging depths using a simultaneous epi/transmissive imaging approach in four different murine tissues; brain, lung, kidney, and liver. For all four cases we report maximum signal sizes, scattering lengths, and achievable imaging depths as a function of tissue type and sample thickness. We report that for murine brain samples thinner than 2 mm transmissive imaging provides better results, while samples 2 mm and thicker are best imaged with epi imaging. We also demonstrate the use of a CNN-based denoising algorithm to yield a 40 µ...Continue Reading

References

Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Conor L EvansX Sunney Xie
Nov 22, 2005·Nature Methods·Fritjof Helmchen, Winfried Denk
Dec 24, 2007·Optics Express·A J WrightX S Xie
Dec 4, 2010·Science·Brian G SaarX Sunney Xie
Nov 24, 2011·Optics Express·Emmanuelle ChaigneauR Angus Silver
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lu WeiWei Min
Mar 13, 2014·Journal of Biomedical Optics·Alba Alfonso-GarcíaEric O Potma
Apr 23, 2014·Angewandte Chemie·Senlian HongXing Chen
May 27, 2014·Journal of Biomedical Optics·Dane M DrutisManoj Misra
May 30, 2014·Accounts of Chemical Research·Delong ZhangJi-Xin Cheng
Jul 18, 2015·Cell·Douglas S Richardson, Jeff W Lichtman
Oct 31, 2015·Annual Review of Biomedical Engineering·Chi ZhangJi-Xin Cheng
May 20, 2016·Cancer Research·Fa-Ke LuNathalie Y R Agar
Dec 29, 2016·Journal of the American Chemical Society·Dan FuXiaoliang Sunney Xie
May 26, 2017·Current Opinion in Chemical Biology·Dan Fu
Jan 4, 2018·Nature Communications·Kimitoshi KimuraTakashi Yamamura
Nov 28, 2018·Nature Methods·Martin WeigertEugene W Myers
Mar 7, 2019·Light, Science & Applications·Yan-Jie ZhaoDan Zhu
Mar 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Mian WeiWei Min
Jul 10, 2019·Analytical Chemistry·Andrew H Hill, Dan Fu
Aug 28, 2019·Biomedical Optics Express·Bryce ManifoldDan Fu
Oct 28, 2019·Biomedical Optics Express·Barbara SarriHerve Rigneault

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Citations

Jan 5, 2021·The Analyst·Martin LeeValerie G Brunton
Jan 19, 2021·The Analyst·Mariko Egawa
May 12, 2021·Journal of Cell Science·Tao ChenMeng C Wang

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