Tissue inhibitor of metalloproteinases-4 suppresses vascular smooth muscle cell migration and induces cell apoptosis

Life Sciences
Yan-Hong GuoKuanghueih Chen

Abstract

In a previous study, we have demonstrated that overexpression of the tissue inhibitors of metalloproteinases-4 (TIMP-4) can inhibit the neointima formation in the rat carotid model. To define the functions of tissue inhibitor of metalloproteinases-4 (TIMP-4) in SMCs, we transduced human TIMP-4 cDNA into rat aortic SMCs by using adenoviral vector. Overexpression of TIMP-4 blocked the conversion of pro-MMP-2 to the active form and inhibited basic fibroblast growth factor-induced migration by 56.7% (p < 0.01). Overexpression of TIMP-4 markedly increased apoptotic cell death without changing their proliferation. Importantly, overexpression of human TIMP-4 in the wall of balloon-injured rat carotid artery also increased SMC apoptosis. The percentages of TUNEL-positive cells of total cells increased significantly in AdTIMP-4 infected group compared with AdGFP infected group. These findings demonstrate that TIMP-4 can inhibit SMCs migration and induce apoptosis in vitro and in vivo, which may generate new targets for prevention and treatment of vascular diseases.

References

Sep 1, 1995·Circulation Research·S M SchwartzE R O'Brien
Nov 29, 1996·The Journal of Biological Chemistry·J GreeneY E Shi
Aug 15, 1997·The Journal of Biological Chemistry·Y E LiuY E Shi
Mar 19, 1999·Circulation Research·C M DolleryY E Shi
Feb 13, 2001·Journal of Cellular Biochemistry·C M TummalapalliS C Tyagi
Sep 17, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mikko P TurunenSeppo Ylä-Herttuala

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Citations

Mar 8, 2006·Histochemistry and Cell Biology·Ilpo KoskivirtaHannu Järveläinen
May 9, 2006·Cancer Metastasis Reviews·Rosemarie ChircoHyeong-Reh Choi Kim
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Shumin BuEnkui Duan
May 2, 2014·Biochemical Pharmacology·Francis G Spinale, Francisco Villarreal
Sep 1, 2014·Toxicology and Applied Pharmacology·Claudia MontaniDiego Di Lorenzo
Jun 24, 2008·Critical Reviews in Clinical Laboratory Sciences·William Cruz-Munoz, Rama Khokha
Jan 27, 2009·Experimental Cell Research·Tabea BingerJochen Ringe
Jun 22, 2006·The Veterinary Clinics of North America. Small Animal Practice·Giselle Hosgood
Mar 28, 2013·Proteomics. Clinical Applications·Nicolle L PattersonMerry L Lindsey
Jan 13, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Christophe F ChantrainEtienne Marbaix
Aug 21, 2015·PloS One·Floria LizarragaJorge Melendez-Zajgla
Sep 15, 2015·PloS One·Srilatha NalluriMumtaz V Rojiani
Jul 28, 2016·Biochimica Et Biophysica Acta·Daniela LigiFerdinando Mannello
Oct 18, 2016·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Flavia Del PortoMaurizio Taurino
Nov 24, 2006·Journal of Dental Research·J Verstappen, J W Von den Hoff
Dec 13, 2017·Clinical and Experimental Hypertension : CHE·Valéria C SandrimRicardo C Cavalli
Feb 3, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M M MurrayA Steinert
Dec 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Neil HendersonSimon Richard Johnson
Jul 14, 2019·Journal of Thrombosis and Haemostasis : JTH·Esther J CookeAnnette von Drygalski
Mar 7, 2007·Expert Review of Cardiovascular Therapy·Jason Lee Johnson
Jan 10, 2021·International Journal of Molecular Sciences·Griselda A Cabral-PachecoMargarita L Martinez-Fierro

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