Tissue microarray analysis of eIF4E and its downstream effector proteins in human breast cancer.

Journal of Experimental & Clinical Cancer Research : CR
Heather E KleinerBenjamin D Li

Abstract

Eukaryotic initiation factor 4E (eIF4E) is elevated in many cancers and is a prognostic indicator in breast cancer. Many pro-tumorigenic proteins are selectively translated via eIF4E, including c-Myc, cyclin D1, ornithine decarboxylase (ODC), vascular endothelial growth factor (VEGF) and Tousled-like kinase 1B (TLK1B). However, western blot analysis of these factors in human breast cancer has been limited by the availability of fresh frozen tissue and the labor-intensive nature of the multiple assays required. Our goal was to validate whether formalin-fixed, paraffin-embedded tissues arranged in a tissue microarray (TMA) format would be more efficient than the use of fresh-frozen tissue and western blot to test multiple downstream gene products. Breast tumor TMAs were stained immunohistochemically and quantitated using the ARIOL imaging system. In the TMAs, eIF4E levels correlated strongly with c-Myc, cyclin D1, TLK1B, VEGF, and ODC. Western blot comparisons of eIF4E vs. TLK1B were consistent with the immunohistochemical results. Consistent with our previous western blot results, eIF4E did not correlate with node status, ER, PR, or HER-2/neu. We conclude that the TMA technique yields similar results as the western blot techniqu...Continue Reading

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Citations

Mar 6, 2010·Analytical and Bioanalytical Chemistry·Marie-Claude DjidjaMalcolm R Clench
Jul 16, 2010·Bioinformatics·Timo ErkkiläHarri Lähdesmäki
Mar 27, 2012·Journal of Clinical Pathology·Christopher FioreRichard Flavin
Jul 10, 2014·Expert Opinion on Therapeutic Targets·Filippa PetterssonWilson H Miller
Aug 6, 2010·Cancer Biology & Therapy·Matthew J WheaterJeremy P Blaydes
Oct 10, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul GreavesJohn G Gribben
May 20, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tsung-Han YehJenn-Yah Yu
Mar 7, 2012·Oncotarget·Jinqiang HouShudong Wang
Dec 12, 2018·European Journal of Medicinal Chemistry·Xinrui YuanHuibin Zhang

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Methods Mentioned

BETA
Cytomation

Software Mentioned

ARIOL
Excel
Intelligent Quantifier
SAS

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