Tissue microarray for routine analysis of breast biomarkers in the clinical laboratory

American Journal of Clinical Pathology
Thomas A ThomsonBryan Knight

Abstract

Tissue microarray analysis (TMA) allows multiple analyses on multiple patients on sections from a single paraffin block. Although it is widely used in research and in quality assurance settings, there are few references to its use in clinical practice. This study evaluated TMA assessment of breast biomarkers using immunohistochemical analysis in a clinical histopathology laboratory. Performance parameters, interobserver variability, and concordance between TMA and whole section results were assessed. The arrays had few lost or noninformative cores. A loss of stain intensity occurred in the arrays compared with the whole sections with some but not all antibodies, highlighting the need to validate the staining protocol for each antibody used on TMA sections. With recommended guidelines for specimen selection and reporting, TMA was found to be an economical replacement for whole section analysis for breast biomarkers.

References

Jul 14, 1998·Nature Medicine·J KononenO P Kallioniemi
Jan 5, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·R L CampD L Rimm
Jan 11, 2001·Advances in Anatomic Pathology·H MochG Sauter
Mar 21, 2001·Human Molecular Genetics·O P KallioniemiG Sauter
Jun 14, 2006·Virchows Archiv : an International Journal of Pathology·Anna SapinoGianni Bussolati
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Feb 13, 2007·Pathology, Research and Practice·Kristof EgervariZoltan Nemes

❮ Previous
Next ❯

Citations

Apr 13, 2010·Molecular & Cellular Proteomics : MCP·Rikke Leth-LarsenHenrik J Ditzel
Oct 31, 2012·Archives of Pathology & Laboratory Medicine·Junya FukuokaLucian R Chirieac
May 18, 2010·American Journal of Clinical Pathology·Thomas A ThomsonBryan Knight
May 20, 2014·Expert Review of Proteomics·Pavel GromovIrina Gromova
Mar 3, 2020·EMBO Molecular Medicine·Lidia Moyano-GalceranKaisa Lehti
Jan 18, 2019·Applied Immunohistochemistry & Molecular Morphology : AIMM·Chih-Hung LinChing-Hsiu Yang
Oct 10, 2015·Advances in Anatomic Pathology·Jeffrey D GoldsmithPaul E Swanson
May 29, 2019·Applied Immunohistochemistry & Molecular Morphology : AIMM·Luiza R GrazziotinDeborah A Marshall
Aug 14, 2019·Virchows Archiv : an International Journal of Pathology·An Sen TanPuay Hoon Tan
Dec 22, 2016·Menopause : the Journal of the North American Menopause Society·Mirette HannaCaroline Diorio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.