Tissue-nonspecific alkaline phosphatase is activated in enterocytes by oxidative stress via changes in glycosylation

Inflammatory Bowel Diseases
R López-PosadasF Sánchez de Medina

Abstract

Intestinal inflammation produces an induction of alkaline phosphatase (AP) activity that is attributable in part to augmented expression, accompanied by a change in isoform, in epithelial cells. This study focuses on induction of AP in intestinal epithelial cells in vitro. Treatment with the oxidants H2O2, monochloramine, or tButOOH increases AP activity in vitro in Caco-2, HT29, and IEC18 cells. We selected IEC18 cells for further testing. Basal AP activity in IEC18 cells is of the tissue-nonspecific (bone-liver-kidney) type, as indicated by Northern and Western blot analysis. Oxidative stress augments AP activity and the sensitivity of the enzyme to levamisole, homoarginine, and heat in IEC18 cells. Increased immunoreactivity to tissue-nonspecific AP antibodies suggests an isoform shift from liver to either kidney or bone type. This effect occurs without changes at the mRNA level and is sensitive to tunicamycin, an inhibitor of N-glycosylation, and neuraminidase digestion. Saponin and deoxycholate produce similar effects to oxidants. Butyrate but not proinflammatory cytokines or LPS can induce a similar effect but without toxicity. The AP increase is not prevented by modulators of the MAPK, NF-κB, calcium, and cyclic adenosin...Continue Reading

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Citations

Sep 4, 2012·European Journal of Nutrition·Borja OcónOlga Martínez-Augustin
Sep 10, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Caroline B AppleyardClaudio De Simone
Nov 16, 2013·The British Journal of Nutrition·Mercedes Ortega-GonzálezOlga Martínez-Augustin
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Dec 19, 2012·International Immunopharmacology·Patricia Martínez-MoyaFermín Sánchez de Medina
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Jul 15, 2015·European Journal of Nutrition·Fermín Capitán-CañadasOlga Martínez-Augustin
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Oct 21, 2016·Inflammatory Bowel Diseases·Rocío López-PosadasFermín Sánchez de Medina
Jul 15, 2018·British Journal of Pharmacology·Carlos J ArandaFermín Sánchez de Medina
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Mar 20, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Min-Seok SeoYong-Jae Lee
Feb 5, 2021·Frontiers in Pediatrics·María Victoria Rodríguez-BenítezOlga Martínez-Augustin
Mar 9, 2021·British Journal of Pharmacology·María Arredondo-AmadorFermín Sánchez de Medina
Jun 17, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Belén Rivero-GutiérrezOlga Martínez-Augustin

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