PMID: 9640669Jun 26, 1998Paper

Tissue-related changes in methyl-esterification of pectic polysaccharides in cauliflower (Brassica oleracea L. var. botrytis) stems

Planta
A FemeniaJ A Robertson

Abstract

Pectic substances are a major component of cell walls in vegetable plants and have an important influence on plant food texture. Cauliflower (Brassica oleracea L. var. botrytis) stem sections at different regions of the mature plant stem have been monitored for tissue-related changes in the native pectic polysaccharides. Chemical analysis detected appreciable differences in the degree of methyl-esterification (ME) of pectic polysaccharides. About 65% of galacturonic acid (GalpA) residues were methyl-esterified in floret tissues. Relative ME showed a basipetal decrease, from 94% in the upper stem to 51% in the lower-stem vascular tissues. The decrease was not related to a basipetal increase in glucuronic acid (GlcpA) residues. The monoclonal antibodies, JIM 5 and JIM 7, produced distinct labelling patterns for the relatively low-methylesterified and high-methyl-esterified pectin epitopes, respectively. Labelling was related to cell type and tissue location in the stem. Floret cell walls contained epitopes for both JIM 5 and JIM 7 throughout the wall. Stem vascular tissues labelled more strongly with JIM 5. Whereas pith parenchyma in the upper stem labelled more strongly with JIM 7, in the lower-stem pith parenchyma, JIM 5 labell...Continue Reading

Citations

May 12, 2011·Applied Microbiology and Biotechnology·Claudia Esperanza TorresAngeles Blanco
Jul 25, 2003·Phytochemistry·B W EvansM C Jarvis
Jul 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·D RogerE Lainé
May 20, 2000·Biotechnology & Genetic Engineering Reviews·R A PradeA J Mort
Oct 12, 1999·Plant Physiology·M W DaveyM C Van Montagu
Apr 12, 2002·FEBS Letters·Kenth JohanssonHans Eklund
Jul 11, 2012·International Journal of Biological Macromolecules·F MunarinP Petrini
Mar 3, 2005·Journal of Agricultural and Food Chemistry·John H Grabber, Ronald D Hatfield
Nov 24, 1999·Journal of Agricultural and Food Chemistry·A FemeniaJ A Robertson

❮ Previous
Next ❯