PMID: 11903800Mar 21, 2002Paper

Tissue shrinkage and unbiased stereological estimation of particle number and size

Journal of Microscopy
Karl-Anton Dorph-PetersenH J Gundersen

Abstract

This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paper introduces new estimators to be used in optical fractionator and optical disector designs; the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.

Citations

Dec 8, 2010·Archives of General Psychiatry·Christine KonradiStephan Heckers
Jan 26, 2005·Experimental Brain Research·J JelsingB Pakkenberg
Nov 16, 2007·Basic Research in Cardiology·Judith Christina EiseleChristian Mühlfeld
Jul 22, 2008·Acta Neuropathologica·Karl-Anton Dorph-PetersenDavid A Lewis
Jun 25, 2008·European Archives of Psychiatry and Clinical Neuroscience·Tomasz GosBernhard Bogerts
May 26, 2005·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Rudolf KarchWolfgang Schreiner
Jun 14, 2005·Hearing Research·Thomas Templin, Andrea Megela Simmons
May 16, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Lucie KubínováJiří Janáček
May 9, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Robert A SweetDavid A Lewis
Oct 26, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Glenn T KonopaskeDavid A Lewis
Jun 19, 2012·Physics in Medicine and Biology·Robert F HobbsGeorge Sgouros
Jul 31, 2009·The American Journal of Surgical Pathology·Rikke Riber-HansenTorben Steiniche
Nov 18, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Kelley BentleyMarc B Hershenson
Apr 26, 2006·Nephron. Experimental Nephrology·John M BasgenJens R Nyengaard
Sep 27, 2003·American Journal of Respiratory and Critical Care Medicine·Matthias OchsHans Jørgen G Gundersen
Feb 5, 2010·American Journal of Respiratory and Critical Care Medicine·Connie C W HsiaUNKNOWN ATS/ERS Joint Task Force on Quantitative Assessment of Lung Structure
Nov 14, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lise LyckHenrik Daa Schrøder
Jan 10, 2013·PloS One·Kresten Krarup KellerEllen-Margrethe Hauge
Jun 21, 2003·Radiation Research·Lucie KubínováJohn O Archambeau
May 7, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·M MyersJ B Kerr
Dec 25, 2013·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Jens R Nyengaard, Saleh H Alwasel
Feb 16, 2013·Cerebral Cortex·Carmen WongStephen G Lomber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.