PMID: 7546295Oct 1, 1995Paper

Tissue-specific alternative promoters regulate the expression of the two sarco/endoplasmic reticulum Ca-ATPase isoforms from Artemia franciscana

DNA and Cell Biology
R Escalante, L Sastre

Abstract

The sarco/endoplasmic reticulum Ca-ATPase gene from Artemia franciscana is transcribed into two mRNAs of 4.5 and 5.2 kb that code for protein isoforms differing at their carboxyl terminus. Northern blot assays and anchored polymerase chain reaction (PCR) experiments have shown that these two mRNAs also differ at the initial part of their 5' untranslated region. The 5.2-kb mRNA-specific 5' untranslated region is present as an independent exon whose transcription is regulated by a promoter different from the one previously described that regulates the expression of the 4.5-kb mRNA. The nucleotide sequence of the 5.2-kb mRNA promoter and the transcription initiation site have been determined. These results suggest that the expression of the two protein isoforms is regulated in A. franciscana at the transcription initiation step, in contrast with the vertebrates sarco/endoplasmic reticulum Ca-ATPase genes 1 and 2 which have unique promoters for transcription of the two isoforms encoded by each gene.

References

Sep 1, 1990·Genes & Development·E N Olson
Jan 1, 1987·Annual Review of Genetics·U Schibler, F Sierra
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Aug 1, 1980·European Journal of Biochemistry·C Osuna, J Sebastián
Mar 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R EscalanteL Sastre

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Citations

Jan 25, 2003·Cell Calcium·F WuytackL Missiaen
Jan 4, 2013·PloS One·Ianina AltshulerMelania E Cristescu
Mar 26, 2003·The Journal of Experimental Biology·Ana Martinez-LampareroLeandro Sastre

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