Tissue-specific expression of a defence-related peroxidase in transgenic wheat potentiates cell death in pathogen-attacked leaf epidermis.

Molecular Plant Pathology
Patrick Schweizer

Abstract

Gene technology can offer creative solutions to problems of agronomical relevance, which may not be solved by conventional breeding methods. One of the major problems of wheat cultivation is disease caused by a number of fungal pathogens including the wheat powdery mildew fungus Blumeria graminis f.sp. tritici (Bgt). Transgenic wheat plants that constitutively express the coding sequence of the defence-related wheat peroxidase TaPrx103 (previously TaPERO) in shoot epidermis under the control of the wheat GstA1 promoter were generated and found to exhibit enhanced resistance to Bgt (Altpeter et al., Plant. Mol. Biol. 57, 271-283). Here, I report on physiological and molecular analyses of these plants in order to assess the mode of action of the peroxidase encoded by the TaGstA1:TaPrx103 transgene. Epidermal cells of transgenic lines with enhanced resistance were found to respond to Bgt attack more frequently with hypersensitive cell death and the generation of hydrogen peroxide. By contrast, resistance of epidermal cell walls to degradation by fungal enzymes appeared to be similar in transgenic and wild-type plants. Moreover, the analysis of the abundance of approximately 10,000 wheat transcripts revealed no significant effect o...Continue Reading

References

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Citations

May 17, 2012·PLoS Pathogens·Christoph HemetsbergerGunther Doehlemann
Aug 7, 2010·Molecular Plant-microbe Interactions : MPMI·Ruth EichmannRalph Hückelhoven
Aug 7, 2010·Molecular Plant-microbe Interactions : MPMI·Reinhard K ProelsRalph Hückelhoven
Aug 7, 2009·Molecular Plant-microbe Interactions : MPMI·Ester SimonettiIsidoro López-Braña
Feb 25, 2010·Molecular Genetics and Genomics : MGG·Gábor KocsyNils Stein
Aug 22, 2009·Molecular Plant Pathology·Mattias PerssonChristina Dixelius
Feb 15, 2011·Journal of Experimental Botany·Eleanor MarshallJose Gutierrez-Marcos
Jul 1, 2020·Frontiers in Plant Science·L Margarita López-CastilloSilverio García-Lara
Sep 28, 2021·Frontiers in Plant Science·Hongbo YuanQian-Hua Shen

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