PMID: 3754047Feb 11, 1986Paper

Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e

Nucleic Acids Research
C J Omiecinski

Abstract

The tissue-specific expression of cytochrome P-450b and P-450e mRNAs was examined with synthetic 18-mer oligomer probes in the liver, lung, kidney, and testis of control and inducer pretreated adult rats. RNAs homologous to the P-450e probe were detected in trace amounts in control and 3-methylcholanthrene (MC) induced livers and at high levels in livers from phenobarbital (PB) induced animals. P-450e mRNA levels were below detection limits in the other tissues examined, regardless of pretreatment. In contrast, mRNAs homologous to the P-450b oligomer were detected at low levels in control and inducer pretreated lung and testis, and at high levels in PB induced liver. No P-450b mRNAs were detected in these assays in RNA isolates from the kidney or from control or MC pretreated liver. Solution hybridization data indicated that the rat lung contained 9-12%, and the testis, 6-9%, respectively, of the levels of P-450b mRNA measured in the PB induced liver. Results from oligo(dT)-cellulose and poly(U)-affinity experiments indicated that the hepatic mRNAs for P-450b and P-450e were present predominantly in the bound, polyadenylated fraction, whereas the homologous lung and testes P-450b mRNAs predominated in the flow-thru fractions.

References

Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y MizukamiY Fujii-Kuriyama
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P M YuanJ E Shively
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A Rampersaud, F G Walz
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G HuezY Cleuter
May 11, 1982·Biochemistry·D J WaxmanC Walsh

❮ Previous
Next ❯

Citations

Dec 1, 1994·The International Journal of Biochemistry·T Nakajima, R S Wang
Jan 1, 1990·Pharmacology & Therapeutics·F J Gonzalez
Dec 1, 1993·Pharmacology & Therapeutics·M J Seaton, E S Vesell
Dec 1, 1994·Toxicology Letters·M G Gadberry, G P Carlson
Apr 6, 2000·The Biochemical Journal·P Honkakoski, M Negishi
Aug 1, 1996·DNA and Cell Biology·Y Park, B Kemper
Jan 2, 1989·European Journal of Biochemistry·G SrivastavaB K May
Jun 1, 1989·Clinical and Experimental Pharmacology & Physiology·B K May, A J Hansen
Jul 29, 1999·American Journal of Respiratory Cell and Molecular Biology·T SkarinR Toftgârd
May 19, 2000·Toxicology Letters·R Suter-EichenbergerM Schlumpf
Jan 12, 2000·Toxicology Letters·R Suter-EichenbergerM Schlumpf
Nov 1, 1987·Archives of Biochemistry and Biophysics·M ChristouC R Jefcoate
Feb 15, 1990·Archives of Biochemistry and Biophysics·C B MarcusC J Omiecinski
Sep 15, 1990·Toxicology and Applied Pharmacology·H FothG F Kahl
Mar 1, 1991·Biochemical Pharmacology·C M Yang, G P Carlson
Oct 1, 1989·Cell Biochemistry and Function·K Rich, A Lodola
Mar 1, 1988·Journal of Cellular Physiology·E G SchuetzP S Guzelian
Jun 30, 1992·Biochemical and Biophysical Research Communications·H SakaiY Kikkawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.