PMID: 9164869May 15, 1997Paper

Tissue-specific regulation of medium-chain acyl-CoA dehydrogenase gene by thyroid hormones in the developing rat

The Biochemical Journal
Fatima DjouadiJean Bastin

Abstract

During development, gene expression of medium-chain acyl-CoA dehydrogenase (MCAD), a nuclear-encoded mitochondrial enzyme that catalyses the first step of medium-chain fatty acid beta-oxidation, is highly regulated in tissues in accordance with fatty acid utilization, but the factors involved in this regulation are largely unknown. To investigate a possible role of thyroid hormones, rat pups were made hypothyroid by the administration of propylthiouracyl to the mother from day 12 of gestation, and their kidneys, heart and liver were removed on postnatal day 16 to determine MCAD mRNA abundance, protein level and enzyme activity. Similar experiments were run in 3,3',5-tri-iodothyronine (T3)-replaced hypothyroid (1 microg of T3/100 g body weight from postnatal day 5 to 15) and euthyroid pups. Hypothyroidism led to an increase in MCAD mRNA abundance in kidney and a decrease in abundance in heart, but had no effect in liver. The protein levels and enzyme activity were lowered in hypothyroid heart and kidney, suggesting that hypothyroidism affects post-transcriptional steps of gene expression in the kidney. All the effects of hypothyroidism were completely reversed in both heart and kidney by T3 replacement. Injection of a single T3 ...Continue Reading

Citations

Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·J J KruegerM A Portman
Mar 18, 2003·Endocrinology·Haidy H A G M van der PuttenTheo J Visser
Apr 29, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Megan J RitterAnthony N Hollenberg
Aug 9, 2002·Journal of Toxicology and Environmental Health. Part a·Kerry A ThuettErnest E Smith
Jul 12, 2002·American Journal of Physiology. Renal Physiology·Fetta OualiJean Bastin
Nov 18, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·L ZhangA Sainsbury
Feb 24, 2018·Nature Reviews. Endocrinology·Rohit A SinhaPaul M Yen
Jun 29, 2007·American Journal of Physiology. Renal Physiology·Jesus DominguezKatherine J Kelly
Nov 14, 1998·The American Journal of Physiology·F OualiJ Bastin
Aug 8, 2019·Thyroid : Official Journal of the American Thyroid Association·Rohit A SinhaPaul M Yen
Feb 1, 2000·Journal of Molecular and Cellular Cardiology·T D ScholzB C Schutte
Dec 9, 2000·Biochemical and Biophysical Research Communications·M J BarreroD Haro
May 25, 2001·Journal of the American Society of Nephrology : JASN·Fatima Djouadi, Jean Bastin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.