Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development

BMC Developmental Biology
Arne K Christensen, Abbie M Jensen

Abstract

The FERM domain containing protein Mosaic Eyes (Moe) interacts with Crumbs proteins, which are important regulators of apical identity and size. In zebrafish, loss-of-function mutations in moe result in defects in brain ventricle formation, retinal pigmented epithelium and neural retinal development, pericardial edema, and tail curvature. In humans and mice, there are two major alternately spliced isoforms of the Moe orthologue, Erythrocyte Protein Band 4.1-Like 5 (Epb4.1l5), which we have named Epb4.1l5long and Epb4.1l5short, that differ after the FERM domain. Interestingly, Moe and both Epb4.1l5 isoforms have a putative C' terminal Type-I PDZ-Binding Domain (PBD). We previously showed that the N' terminal FERM domain in Moe directly mediates interactions with Crumbs proteins and Nagie oko (Nok) in zebrafish, but the function of the C'terminal half of Moe/Epb4.1l5 has not yet been examined. To define functionally important domains in zebrafish Moe and murine Epb4.1l5, we tested whether injection of mRNAs encoding these proteins could rescue defects in zebrafish moe- embryos. Injection of either moe or epb4.1l5long mRNA, but not epb4.1l5short mRNA, could rescue moe- embryonic defects. We also tested whether mRNA encoding C' ter...Continue Reading

References

Dec 15, 1996·Developmental Biology·S S Easter, G N Nicola
Mar 17, 1999·The Journal of Clinical Investigation·A S Fanning, J M Anderson
Oct 3, 1999·Nature Genetics·A I den HollanderA A Bergen
Mar 20, 2001·Archives of Ophthalmology·A J LoteryE M Stone
Oct 10, 2001·Current Biology : CB·S Horne-BadovinacS Abdelilah-Seyfried
Jun 19, 2002·Development Genes and Evolution·Kevin B Hoover, Peter J Bryant
Apr 18, 2003·Human Molecular Genetics·Samuel G JacobsonEdwin M Stone
Jun 12, 2003·Developmental Biology·James M Fadool
Aug 2, 2003·American Journal of Physiology. Renal Physiology·Michael H Roh, Ben Margolis
Dec 30, 2004·Investigative Ophthalmology & Visual Science·Oliver RinnerStephan C F Neuhauss
Nov 4, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chieko KoikeTakahisa Furukawa
Jan 18, 2006·BMC Developmental Biology·Vinzenz LinkCarl-Philipp Heisenberg
Mar 11, 2006·Neuron Glia Biology·Xiaofei WangMonica M Jablonski
Jan 16, 2007·Molecular and Cellular Neurosciences·Shuang CuiBrian A Link
Oct 9, 2007·Experimental Cell Research·Ilse GosensRonald Roepman
Jul 19, 2008·Neuron Glia Biology·Xiaofei WangMonica M Jablonski
Sep 8, 2010·Bone·Michael DoubeSandra J Shefelbine

❮ Previous
Next ❯

Citations

Jan 25, 2012·Molecular Medicine·Andrea HoffmannPanagiotis A Tsonis
Jul 15, 2009·Current Opinion in Genetics & Development·Ulrich Tepass
Sep 27, 2008·Developmental Biology·Chantilly MunsonDidier Y R Stainier
Oct 30, 2013·Molecular and Cellular Biology·Eileen L WhitemanBen Margolis
Jun 6, 2020·Journal of Cell Science·Tiffany Yu, Miho Matsuda
Dec 5, 2019·Genes·Peter M J Quinn, Jan Wijnholds
Apr 21, 2017·Frontiers in Neuroscience·Peter M QuinnJan Wijnholds
Mar 1, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mi-Hyeon JeongHo-Geun Yoon
Jan 28, 2010·Journal of Cell Science·Hiroyuki Nakajima, Takuji Tanoue

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
transgenic

Software Mentioned

Optomotor
ImageJ
Sync Measure
ClustalX

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.