Tissue-specific transcriptional profiling of iron-deficient and cadmium-stressed rice using laser capture microdissection

Plant Signaling & Behavior
Yuko OgoNaoko K Nishizawa

Abstract

Several metals are essential nutrients for plants. However, they become toxic at high levels and deleteriously affect crop yield and quality. We recently reported the spatial gene expression profiles of iron (Fe)-deficient and cadmium (Cd)-stressed rice using laser microdissection and microarray analysis. The roots of Fe-deficient and Cd-stressed rice were separated into the vascular bundle (VB), cortex (Cor), and epidermis plus exodermis (EP). In addition, vascular bundles from new and old leaves at the lowest node, which are important for metal distribution, were analyzed separately (newDC and oldDC, respectively). Genes expressed in a tissue-specific manner in the VB, Cor, EP, newDC, and oldDC formed large clusters. The genes upregulated in all of the VB, Cor, and EP by Fe deficiency formed a substantial cluster that was smaller than the tissue-specific clusters. Significant numbers of genes expressed in newDC or oldDC were also expressed in VB in roots, suggesting that vascular bundles in the lowest nodes and roots have a partially common function. The expression patterns of transporter families involved in metal homeostasis were investigated, and members of each family were either expressed differentially in each tissue or...Continue Reading

References

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May 9, 2002·Journal of Experimental Botany·Andres Schützendübel, Andrea Polle
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Yuko OgoNaoko K Nishizawa
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Takanori KobayashiNaoko K Nishizawa
Dec 16, 2010·The Journal of Biological Chemistry·Tomoko NozoyeNaoko K Nishizawa
Mar 1, 2012·Scientific Reports·Yasuhiro IshimaruNaoko K Nishizawa
May 17, 2012·The Plant Cell·Akimasa SasakiJian Feng Ma
Nov 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Satoru IshikawaHiromi Nakanishi
Nov 6, 2013·The New Phytologist·Yuko OgoNaoko K Nishizawa

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Citations

Dec 9, 2014·Plant Signaling & Behavior·Abu Baker SiddiqueNarendra Tuteja
Jan 31, 2019·Plants·Anumalla MahenderJauhar Ali
Mar 21, 2020·Scientific Reports·Martin BeckerManfred Karl Adolf Schenk

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