Tissue-specific transcriptional regulation and metabolite accumulation in tomato (Solanum lycopersicum L.).

Protoplasma
Deepak SharmaManoj K Dhar

Abstract

Tomato is an excellent model for studying fruit development, ripening, and other secondary metabolic pathways such as carotenoid biosynthetic pathway, flavonoid pathway, and many more. Tomato fruit development and ripening occurs under tight genetic control and involves the expression of thousands of genes affecting fruit quality and accumulation of pigments and metabolites. Here, we have described the development of a microarray platform that has allowed establishment of a framework for quantification of the expression of large number of genes and transcription factors possibly regulating various secondary metabolic pathways in tomato. To unravel the molecular mechanisms of fruit development and ripening, a tomato 60-mer oligonucleotide 44 K microarray along with the custom array for many genes and transcription factors was designed and validated in the fruit and leaf tissues. Comparative profiling of gene expression studies has allowed us to identify a large number of differentially expressed genes and transcription factors. Gene ontology revealed the involvement of these genes in various biological, cellular, and molecular processes like isoprenoid, terpenoid, pigment, ethylene biosynthesis, phytohormone signaling, and fruit...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 27, 2002·Journal of Experimental Botany·Lucille Alexander, Don Grierson
Sep 27, 2002·Journal of Experimental Botany·Peter M Bramley
Aug 9, 2003·BMC Plant Biology·Glenn E Bartley, Betty K Ishida
Sep 27, 2005·Plant Physiology·Martine Lemaire-ChamleyChristophe Rothan
Feb 2, 2006·Journal of Experimental Botany·Fernando Carrari, Alisdair R Fernie
Apr 20, 2007·Current Opinion in Plant Biology·James J Giovannoni
Apr 10, 2008·The Plant Journal : for Cell and Molecular Biology·Zhefeng LinDon Grierson
Nov 6, 2008·Archives of Biochemistry and Biophysics·John W ErdmanBrian L Lindshield
Nov 7, 2009·The Plant Journal : for Cell and Molecular Biology·Maxim ItkinAsaph Aharoni
Dec 8, 2009·Trends in Plant Science·Mar Martín-Trillo, Pilar Cubas
Mar 26, 2010·Journal of Experimental Botany·Irvin L PanVivian F Irish
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gabriela Toledo-OrtizManuel Rodríguez-Concepción
May 4, 2011·Journal of Food Science·Mathilde CausseSilvana Grandillo
Nov 9, 2011·Annual Review of Genetics·Harry J Klee, James J Giovannoni
Jan 1, 2010·Annual Review of Food Science and Technology·Erica N StoryG Keith Harris
May 10, 2012·Molecular Genetics and Genomics : MGG·Shengxiong HuangYongsheng Liu
Jun 21, 2013·Frontiers in Plant Science·Sonia OsorioAlisdair R Fernie
Dec 25, 2013·Journal of Microbiological Methods·Balaji VenkataramanAmita Gupta
Feb 21, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Amanda S Berenschot, Vera Quecini
Jun 7, 2014·BMC Plant Biology·Violeta ParapunovaRuud A de Maagd
Jun 12, 2014·Briefings in Functional Genomics·Manoj K DharSanjana Kaul
Aug 1, 2014·Journal of Experimental Botany·Rumyana KarlovaRuud A de Maagd
Apr 1, 2013·Biomolecular Concepts·Nora G Uberti ManasseroDaniel H Gonzalez

❮ Previous
Next ❯

Citations

May 29, 2021·BMC Plant Biology·Sara ForlaniSimona Masiero

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE11277

Methods Mentioned

BETA
chip
Feature Extraction
PCR

Software Mentioned

array
Agilent Feature Extraction
Cytoscape
GeneSpring GX
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.