Tissue-specific transgenic knockdown of Fos-related antigen 2 (Fra-2) expression mediated by dominant negative Fra-2

Molecular and Cellular Biology
M SmithR Baler

Abstract

Fos-related antigen 2 (Fra-2) is a member of the Fos family of immediate-early genes, most of which are rapidly induced by second messengers. All members of this family act by binding to AP-1 sites as heterodimeric complexes with other proteins. However, each appears to have a distinct role. The role and biology of Fra-2 are less well understood than those of its relatives c-Fos, Fra-1, and FosB; moreover, Fra-2 target genes remain largely unknown, as does the basis of its selective effects on transcriptional activity. To pursue these issues, we created a transgenic rat line (NATDNF2) in which a dominant negative fra-2 (DNF2) gene is strongly expressed in the pineal gland; tissue selectivity was achieved by putting the DNF2 gene under the control of the rat arylalkylamine N-acetyltransferase (AANAT) regulatory region, which targets gene expression to a very restricted set of tissues (pineal gland > retina). Expression of AANAT is normally turned on after the onset of darkness in the rat; as a result, pineal DNF2 expression occurs only at night. This was associated with marked suppression of the nocturnal increase in fra-2 mRNA and protein levels, indicating that DNF2 expression inhibits downstream effects of Fra-2, including th...Continue Reading

References

Jul 1, 1992·International Journal of Peptide and Protein Research·F Baleux, P Dubois
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Hai, T Curran
Oct 25, 1991·Nucleic Acids Research·T SuzukiH Iba
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H NishinaH Iba
Nov 10, 1995·The Journal of Biological Chemistry·R Baler, D C Klein
Jun 11, 1993·Nucleic Acids Research·T YoshidaH Iba
Jan 11, 1994·Nucleic Acids Research·C T van OostromH van Steeg
Apr 1, 1996·Immunology and Cell Biology·V C Foletta
May 19, 1997·Biochemical and Biophysical Research Communications·M SammarP Altevogt
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·D KrylovC Vinson
Dec 24, 1997·Journal of Pineal Research·Z Trávnícková, H Illnerová
Oct 24, 1998·Genes & Development·J MoitraC Vinson
May 13, 1999·The Journal of Biological Chemistry·D GuptaR Dziarski
Aug 14, 1999·Journal of Neuropathology and Experimental Neurology·V SennerW Paulus

❮ Previous
Next ❯

Citations

Jun 5, 2003·European Journal of Pharmacology·Rae R MatsumotoDaniel J Brackett
Aug 2, 2001·Journal of Neuroscience Methods·T Wells, D A Carter
Apr 3, 2003·Brain Research. Molecular Brain Research·Elena DiazValérie Simonneaux
Oct 3, 2002·Journal of Neurochemistry·F GuillaumondA M François-Bellan
Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Balázs Gereben, Domenico Salvatore
Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Françoise CourtinMichel Pierre
Mar 22, 2007·Trends in Endocrinology and Metabolism : TEM·Erik Maronde, Jörg H Stehle
Jul 8, 2008·Journal of Neuroendocrinology·K J Kovács
Oct 29, 2009·Journal of Neurochemistry·Anthony K Ho, Constance L Chik
Jun 29, 2004·Biochemical and Biophysical Research Communications·Ann Humphries, David A Carter
May 2, 2006·Molecular and Cellular Endocrinology·J S Davies, D A Carter
Jun 28, 2011·Molecular and Cellular Endocrinology·Constance L ChikAnthony K Ho
Feb 14, 2012·Chronobiology International·F GuillaumondA M François-Bellan
May 12, 2006·Chronobiology International·Valérie SimonneauxPaul Pévet
Oct 4, 2016·Journal of Agricultural and Food Chemistry·Yifei WangNicholi Vorsa
Oct 27, 2004·The Journal of Biological Chemistry·Jong-So KimDavid C Klein
Jun 29, 2002·Molecular Endocrinology·Balázs GerebenP Reed Larsen
Jul 19, 2003·Chronobiology International·Estela Muñoz, Ruben Baler
Sep 26, 2008·Endocrine Reviews·Balázs GerebenAntonio C Bianco
Mar 4, 2014·Clinical and Experimental Otorhinolaryngology·Seung Woo KimKee Hwan Kwon
Jun 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Makoto MatsumotoAlan Aderem
Aug 10, 2004·The Journal of Biological Chemistry·Weizhong ChangThomas L McCarthy
Apr 24, 2021·The Journal of Pain : Official Journal of the American Pain Society·Matthew R SapioAndrew J Mannes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.