Abstract
Amorpha-4,11-diene synthase (ADS) of Artemisia annua L. is a sesquiterpene cyclase that catalyzes the conversion of farnesyl diphosphate into amorpha-4,11-diene in the biosynthesis of the antimalarial artemisinin. To explore the mechanisms regulating the tissue-specific and developmental distributions of ADS, a full ADS promoter was generated using PCR, and fused to GUS for introduction into Arabidopsis thaliana. ADSpro::GUS fusion transcripts were organ-specific, mainly present in the anthers and trichomes of the green tissues of the juvenile leaves. This result was consistent with the ADS transcription pattern observed in A. annua as examined by RT-PCR. To determine the subcellular localization of ADS, an open reading frame (ORF) of ADS was fused to the green fluorescent protein (smGFP) gene and introduced into the A. thaliana protoplasts. GFP fluorescence was located exclusively in the cytosol, an indication that ADS is a cytosol-localized protein.
Citations
Oct 23, 2012·Plant Cell Reports·Khanhvan T NguyenPamela J Weathers
May 27, 2011·Planta·Fatima Alejos-GonzalezDe-Yu Xie
Jun 15, 2011·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Khanhvan T NguyenPamela J Weathers
Nov 3, 2009·Plant & Cell Physiology·Dongming MaBenye Liu
Jan 15, 2014·Plant Cell Reports·Kexuan TangXueqing Fu
Jun 28, 2014·Plant & Cell Physiology·Yunpeng JiHong Wang
Apr 30, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Patrick R ArsenaultPamela J Weathers
Feb 13, 2016·The New Phytologist·Qian ShenKexuan Tang
Sep 5, 2015·Plant Signaling & Behavior·Sunita JindalVikrant Gupta
Apr 12, 2016·BioMed Research International·Weimin JiangKexuan Tang
Feb 10, 2016·Plant & Cell Physiology·Zongyou LvKexuan Tang
Dec 15, 2010·Plant Biology·S CarettoG Mita
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Alain Tissier
Nov 23, 2011·Molecular Plant·Zong-Xia YuXiao-Ya Chen
Mar 31, 2012·Trends in Plant Science·Nathan De GeyterAlain Goossens
Jun 3, 2016·Journal of Natural Medicines·Paskorn MuangphromToshiya Muranaka
Aug 6, 2019·Frontiers in Plant Science·Pearlin Shabna NazizSupriyo Sen
May 31, 2011·Phytochemistry·Mohammad MajdiHarro Bouwmeester