Tissue specificity and developmental pattern of amorpha-4,11-diene synthase (ADS) proved by ADS promoter-driven GUS expression in the heterologous plant, Arabidopsis thaliana

Planta medica
Soon-Hee KimSoo-Un Kim

Abstract

Amorpha-4,11-diene synthase (ADS) of Artemisia annua L. is a sesquiterpene cyclase that catalyzes the conversion of farnesyl diphosphate into amorpha-4,11-diene in the biosynthesis of the antimalarial artemisinin. To explore the mechanisms regulating the tissue-specific and developmental distributions of ADS, a full ADS promoter was generated using PCR, and fused to GUS for introduction into Arabidopsis thaliana. ADSpro::GUS fusion transcripts were organ-specific, mainly present in the anthers and trichomes of the green tissues of the juvenile leaves. This result was consistent with the ADS transcription pattern observed in A. annua as examined by RT-PCR. To determine the subcellular localization of ADS, an open reading frame (ORF) of ADS was fused to the green fluorescent protein (smGFP) gene and introduced into the A. thaliana protoplasts. GFP fluorescence was located exclusively in the cytosol, an indication that ADS is a cytosol-localized protein.

Citations

Jun 15, 2011·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Khanhvan T NguyenPamela J Weathers
Jan 15, 2014·Plant Cell Reports·Kexuan TangXueqing Fu
Apr 30, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Patrick R ArsenaultPamela J Weathers
Apr 12, 2016·BioMed Research International·Weimin JiangKexuan Tang
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Alain Tissier
Mar 31, 2012·Trends in Plant Science·Nathan De GeyterAlain Goossens
Aug 6, 2019·Frontiers in Plant Science·Pearlin Shabna NazizSupriyo Sen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.