Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation.

PloS One
Asako ShindoNaoto Ueno

Abstract

The establishment of cell polarity is crucial for embryonic cells to acquire their proper morphologies and functions, because cell alignment and intracellular events are coordinated in tissues during embryogenesis according to the cell polarity. Although much is known about the molecules involved in cell polarization, the direct trigger of the process remains largely obscure. We previously demonstrated that the tissue boundary between the chordamesoderm and lateral mesoderm of Xenopus laevis is important for chordamesodermal cell polarity. Here, we examined the intracellular calcium dynamics during boundary formation between two different tissues. In a combination culture of nodal-induced chordamesodermal explants and a heterogeneous tissue, such as ectoderm or lateral mesoderm, the chordamesodermal cells near the boundary frequently displayed intracellular calcium elevation; this frequency was significantly less when homogeneous explants were used. Inhibition of the intracellular calcium elevation blocked cell polarization in the chordamesodermal explants. We also observed frequent calcium waves near the boundary of the dorsal marginal zone (DMZ) dissected from an early gastrula-stage embryo. Optical sectioning revealed that w...Continue Reading

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Citations

Jan 5, 2011·PloS One·Michelangelo von DassowLance A Davidson
Jul 14, 2011·Physical Biology·Michelangelo von Dassow, Lance A Davidson
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomLoren L Looger
Sep 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·YongTae KimPhilip R LeDuc
Mar 14, 2013·Development, Growth & Differentiation·Chiyo TakagiNaoto Ueno
Mar 15, 2012·Seminars in Cell & Developmental Biology·Olga Markova, Pierre-François Lenne
Dec 19, 2017·Chemosensors·Zhijie ChenHui-Wang Ai
Sep 15, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Asako Shindo
Nov 12, 2013·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Masamichi Ohkura, Junichi Nakai
Jan 24, 2018·Journal of Cell Science·Hana HříbkováYuh-Man Sun
Nov 2, 2018·International Journal of Molecular Sciences·Sudip PaudelMargaret Saha

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Methods Mentioned

BETA
antisense oligonucleotide
PCR

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