Tissue transglutaminase was up-regulated by EGF-retinoid interplay in epithelial carcinoma cells

Life Sciences
D CaccamoR Ientile

Abstract

Retinoids have been shown the most powerful inducers of transglutaminase activity, a well known marker of differentiation. In this work, we tested the effects of all-trans retinoic acid and EGF, used alone or in combination, on transglutaminase activity in a squamous, epithelial carcinoma cell line, HEp-2. We demonstrated that nanomolar EGF further enhances transglutaminase activity previously induced by all-trans retinoic acid. Confocal laser scanning microscopy revealed functional changes in transglutaminase activity localisation, at first restricted to the outermost region of cytosol, then diffused both in the membrane region and extracellular space. RT-PCR showed the presence of mRNA transcripts of different transglutaminases (1, 2, 3). Transglutaminase 2 expression was increased by either all-trans retinoic acid or EGF, and further up-regulated by the simultaneous addition of both substances. These effects were confirmed by Western blotting with transglutaminase 2 specific antibody. The results obtained by combined use of retinoic acid and EGF suggest that transglutaminase activity and expression are differently regulated, and that EGF-signalling can be involved in differentiation of epithelial carcinoma cells induced by r...Continue Reading

References

Sep 1, 1993·Journal of Cellular Physiology·R L Konger, T C Chan
May 1, 1997·Archives of Dermatological Research·M PonecA M Mommaas
Jan 27, 1998·The Journal of Biological Chemistry·J ZhangG V Johnson
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, K Mehta
Feb 23, 2000·The Journal of Cell Biology·S S AkimovA M Belkin
Jul 8, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·S GibbsM Ponec
Jul 10, 2001·The Journal of Biological Chemistry·E CandiG Melino

❮ Previous
Next ❯

Citations

Feb 7, 2009·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·L ElliD Schuppan
May 12, 2005·The Journal of Clinical Endocrinology and Metabolism·Maryam Kabir-SalmaniMitsutoshi Iwashita

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
Soo-Youl KimPeter M Steinert
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Dong-Myung ShinIn-Gyu Kim
Biochemical and Biophysical Research Communications
Oliver SoehnleinChristoph D Garlichs
© 2021 Meta ULC. All rights reserved