Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro

International Journal of Molecular Medicine
Yu-Wei GeYuanqing Mao

Abstract

The present study investigated the role of bidirectional ephrin‑B2/erythropoietin‑producing human hepatocellular receptor 4 (ephB4) signaling in the regulation of wear particle‑mediated osteoclastogenesis in vitro. Mouse bone marrow macrophages (BMMs) were induced into osteoclasts by receptor activator of nuclear factor‑κB ligand (RANKL, 50 ng/ml). EphB4‑Fc, an osteoblast membrane surface receptor (4 µg/ml), was used to stimulate the ephrin‑B2 ligand of osteoclasts in the presence and absence of titanium (Ti). Tartrate‑resistant acid phosphatase (TRAP) staining was used to detect the number of osteoclasts, and phalloidin staining was used to examine the cytoskeletons of the osteoclasts. A bone pit absorption experiment was used to measure osteoclast function. Reverse transcription quantitative polymerase chain reaction and western blot analysis were used to examine osteoclastogenesis. ELISAs were used to detect the production of inflammatory factors. The data demonstrated that Ti significantly promoted the differentiation of BMMs into mature osteoclasts in the presence of RANKL and significantly promoted expression of the ephrin‑B2, nuclear factor of activated T‑cells 1 (NFATc1), TRAP, Fos proto‑oncogene, AP‑1 transcription fac...Continue Reading

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Citations

Jun 9, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Mi HuangRui Peng
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Agnieszka Arthur, Stan Gronthos

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Methods Mentioned

BETA
light microscopy
PCR
ELISA

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