Titanium Surface Priming with Phase-Transited Lysozyme to Establish a Silver Nanoparticle-Loaded Chitosan/Hyaluronic Acid Antibacterial Multilayer via Layer-by-Layer Self-Assembly

PloS One
Xue ZhongChangyi Li

Abstract

The formation of biofilm around implants, which is induced by immediate bacterial colonization after installation, is the primary cause of post-operation infection. Initial surface modification is usually required to incorporate antibacterial agents on titanium (Ti) surfaces to inhibit biofilm formation. However, simple and effective priming methods are still lacking for the development of an initial functional layer as a base for subsequent coatings on titanium surfaces. The purpose of our work was to establish a novel initial layer on Ti surfaces using phase-transited lysozyme (PTL), on which multilayer coatings can incorporate silver nanoparticles (AgNP) using chitosan (CS) and hyaluronic acid (HA) via a layer-by-layer (LbL) self-assembly technique. In this study, the surfaces of Ti substrates were primed by dipping into a mixture of lysozyme and tris(2-carboxyethyl)phosphine (TCEP) to obtain PTL-functionalized Ti substrates. The subsequent alternating coatings of HA and chitosan loaded with AgNP onto the precursor layer of PTL were carried out via LbL self-assembly to construct multilayer coatings on Ti substrates. The results of SEM and XPS indicated that the necklace-like PTL and self-assembled multilayer were successfull...Continue Reading

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Citations

Mar 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenjing Song, Shaohua Ge
Oct 23, 2019·Journal of Periodontal Research·Erica D de AvilaJeroen J J P van den Beucken
Jul 11, 2020·Journal of Biomaterials Science. Polymer Edition·Liangrui ChenXueqi Gan
Sep 27, 2018·Materials·Jérôme Frédéric LasserreSelena Toma
Dec 14, 2017·PloS One·Mélanie D'AlmeidaBrigitte Grosgogeat
Jan 1, 2020·Carbohydrate Polymers·Mahdi RahimiHossein Samadi Kafil
Feb 14, 2021·Biology·Xinzhen FanEdward Sacher
Aug 25, 2021·European Journal of Dentistry·Diane Isabel SelvidoNatthamet Wongsirichat
Aug 29, 2021·Pharmaceuticals·Gustavo MoraesWalter L Siqueira

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Methods Mentioned

BETA
atomic emission spectrometry
X-ray
scanning electron microscopy
transmission electron microscopy
protein assay
fluorescence microscopy

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