PMID: 11927678Apr 3, 2002Paper

Titin-based contribution to shortening velocity of rabbit skeletal myofibrils

The Journal of Physiology
Ave MinajevaWolfgang A Linke

Abstract

The shortening velocity of skeletal muscle fibres is determined principally by actomyosin cross-bridges. However, these contractile elements are in parallel with elastic elements, whose main structural basis is thought to be the titin filaments. If titin is stretched, it may contribute to sarcomere shortening simply because it can recoil 'passively'. The titin-based contribution to shortening velocity (V(p)) was quantified in single rabbit psoas myofibrils. Non-activated specimens were rapidly released from different initial sarcomere lengths (SLs) by various step amplitudes sufficient to buckle the myofibrils; V(p) was calculated from the release amplitude and the time to slack reuptake. V(p) increased progressively (upper limit of detection, approximately 60 microm s(-1) sarcomere(-1)) between 2.0 and 3.0 microm SL, albeit more steeply than passive tension. At very low passive tension levels already (< 1-2 mN mm(-2)), V(p) could greatly exceed the unloaded shortening velocity measured in fully Ca(2+)-activated skinned rabbit psoas fibres. Degradation of titin in relaxed myofibrils by low doses of trypsin (5 min) drastically decreased V(p). In intact myofibrils, average V(p) was faster, the smaller the release step applied. Al...Continue Reading

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Citations

Feb 8, 2011·European Journal of Applied Physiology·Frédéric DierickEtienne Masquelier
Oct 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christiane A OpitzWolfgang A Linke
Aug 26, 2006·The Journal of Physiology·M Charlotte OlssonLars Larsson
Jul 9, 2005·The Journal of Physiology·Kazushige Sasaki, Naokata Ishii
Mar 9, 2010·Journal of Theoretical Biology·Sefi Givli
Apr 8, 2009·Journal of Theoretical Biology·Urs StoeckerJachen Denoth
Jun 18, 2002·Journal of Structural Biology·Wolfgang A LinkeJulio M Fernandez
Jun 1, 2005·Journal of Biomechanics·Richard M LoveringPatrick G De Deyne
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·A Y Khapchaev, V P Shirinsky
Dec 24, 2017·Scandinavian Journal of Medicine & Science in Sports·N BeaumatinS Dorel
Sep 28, 2004·Physics in Medicine and Biology·Wolfgang A Linke, Mark C Leake
Jul 1, 2006·Muscle & Nerve·Jeffrey J Widrick, Tyler Barker
Nov 14, 2017·Annual Review of Physiology·Wolfgang A Linke
Feb 13, 2018·Annual Review of Physiology·Edward C EckelsJulio M Fernández

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