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Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

Nature Biotechnology

Jan 15, 2020

Marco JostJonathan S Weissman

Abstract

A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we describe an approach to titrate expression of human genes using CRISPR interference and series of single-guide RNAs (sgRNAs) with sys...read more

Mentioned in this Paper

Genome
Protein Expression
Cell Growth
Site
Biochemical Pathway
cDNA Library
RNA
Genes
Modulated
Clustered Regularly Interspaced Short Palindromic Repeats
484
1
1
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Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens

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© 2020 Meta ULC. All rights reserved

Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

Nature Biotechnology

Jan 15, 2020

Marco JostJonathan S Weissman

PMID: 31932729

DOI: 10.1038/s41587-019-0387-5

Abstract

A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we describe an approach to titrate expression of human genes using CRISPR interference and series of single-guide RNAs (sgRNAs) with sys...read more

Mentioned in this Paper

Genome
Protein Expression
Cell Growth
Site
Biochemical Pathway
cDNA Library
RNA
Genes
Modulated
Clustered Regularly Interspaced Short Palindromic Repeats
484
1
1

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CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Perturb-seq

Perturb-seq has combined CRISPR gene inactivation and single-cell rna-sequencing (scRNA-seq) and is the newest addition to the geneticist's arsenal, providing scientists with study methods for functional genomics. Discover the latest research here.

Related Papers

Nature Biotechnology

Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens

Nature BiotechnologyDecember 19, 2017
Fadi J NajmJohn G Doench
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