PMID: 9430197Jul 1, 1997Paper

Titration calorimetry study of an anti-idiotypic antibody cascade in a human melanoma-associated antigen system

Molecular Immunology
M MutzG Pluschke

Abstract

The thermodynamic parameters of interactions between six variants of the anti-idiotypic monoclonal antibody (mAb) CGP 60686 produced by the hybridoma MK2-23 with an idiotypic mAb and five different anti-anti-idiotypic mAb were studied with high sensitivity titration calorimetry. CGP 60686 recognizes an epitope in the antigen-combining region of the human high-molecular-weight-melanoma-associated antigen (HMW-MAA)-specific mouse mAb CGP 76873 produced by the hybridoma 763.74. The five HMW-MAA-specific anti-anti-idiotypic mAbs GH 464, GH 518, GH 149, GH 386 and GH 586 were generated from mice immunization with mAb CGP 60686. All interactions between the anti-idiotypic mAb and the idiotypic mAb or the anti-anti-idiotypic mAb showed large exothermic binding enthalpies between -15 and -23 kcal/mol and binding affinities larger than 6 x 10(9) M-1. Four of the five anti-anti-idiotypic mAbs tested exhibited significantly higher binding enthalpies for the interaction with the anti-idiotypic than the idiotypic mAbs. Replacement of either the heavy or the light chain variable region of the anti-idiotypic mAbs with an unrelated variable region abolished the idiotype to anti-idiotype interaction. Thus, both the heavy and the light chain var...Continue Reading

References

Aug 8, 1991·Nature·J Foote, C Milstein
Sep 1, 1989·The Journal of Clinical Investigation·F Perosa, S Ferrone
Oct 1, 1984·Molecular Immunology·G RialdiF Manca
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R C Mulligan, P Berg
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·T N BhatR J Poljak
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T FulpiusS Izui
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·D R Davies, G H Cohen
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·G PluschkeR A Reisfeld

❮ Previous
Next ❯

Citations

Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

❮ Previous
Next ❯