PMID: 6159857Aug 1, 1980Paper

Titration method for determination of carcinine (beta-alanyl-histamine). Application to the heart of Carcinus maenas (L.)

Archives internationales de physiologie et de biochimie
J M Arnould, C Tankosic

Abstract

1.--Carcinine was isolated by ion-exchange chromatography and titrated spectrophotometrically. The technique used was based on formation of an azo derivative of carcinine with sulfanilamide at basic pH. It allowed detection of carcinine at 1 microgram/ml concentration. 2.--Histamine could be detected simultaneously with the same technique, provided its concentration was greater than 1 microgram/ml. 3.--The mean amount of carcinine in cardiac tissues of Carcinus maenas was 22.1 micrograms/ml dry cardiac tissue. 4.--The technique described above did not allow detection of histamine in the heart of Carcinus maenas. If present, histamine must be at a concentration smaller than 0.1 microgram/ml dry cardiac tissue.

References

Jul 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·D E FisherH Fisher
May 1, 1970·Comparative Biochemistry and Physiology·K G Crush
Jan 1, 1921·The Biochemical Journal·W M Clifford
Jan 1, 1928·The Biochemical Journal·W M Clifford, V H Mottram

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