Apr 29, 2020

Titration of in-cellula affinities of protein-protein interactions

BioRxiv : the Preprint Server for Biology
D. CluetMartin Spichty

Abstract

A genetic assay permits simultaneous quantification of two interacting proteins and their bound fraction at the single-cell level using flow cytometry. In-cellula affinities of protein-protein interactions can be extracted from the acquired data through a titration-like analysis. The applicability of this approach is demonstrated on a diverse set of interactions with proteins from different families and organisms and with in-vitro dissociation constants ranging from picomolar to micromolar.

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Mentioned in this Paper

Computer Software
Genome
Sequence Determinations, RNA
Environment
Research Personnel
Nucleic Acid Sequencing
National Cancer Institute
Sorting - Cell Movement
Sequencing
Tumor Cells, Malignant

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