TK6 genome profile compared with WIL2-NS: Reference data to improve the reproducibility of genotoxicity studies.

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Fumio KasaiArihiro Kohara

Abstract

The TK6 cell line has been widely used for genotoxicity screening by taking advantage of the TK1 mutations. A number of publications have employed TK6 as a tool for the assay; however, its characterization is limited. Because genomes of cell lines are often changed during cell culture, differences in TK6 genomes could be observed between laboratories, which would cause potential problems with reproducibility using TK6. In this study, TK6(IVGT) (JCRB1435), registered with the JCRB Cell Bank as the standard for TK6, has been characterized by karyotyping, SNP microarray and targeted sequencing, and were then compared to WIL2-NS (JCRB9063), a subline derived from a common ancestor with TK6. Changes at the chromosome level were quantitatively assessed by the microarray data, which were shown by DNA sizes and Scales of Genomic Alterations (SGA). An extinct common ancestral cell line, WI-L2 originated from hereditary spherocytosis, has been extrapolated from analysis of genomic signatures shared between TK6 and WIL2-NS, revealing a point mutation in SPTA1, related to the disease. DNA size is calculated to be 102.6 %, 103.1 % and 103.9 % for WI-L2, TK6(IVGT) and WIL2-NS respectively, with the reference values of a normal diploid genome...Continue Reading

References

Sep 1, 1968·Cancer·J A LevyV Defendi
Jun 1, 1982·Mutation Research·H L Liber, W G Thilly
Feb 3, 2005·Environmental and Molecular Mutagenesis·Masamitsu Honma
Apr 19, 2011·Nature Genetics·Xiaomu WeiYardena Samuels
Jan 18, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Javier RevolloVasily N Dobrovolsky
Oct 4, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·E LorgeJ Y Tanir
Feb 7, 2018·Acta Haematologica·Ben-Jin HeFa-Quan Lin

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