TLC characterization of liposomes containing angiotensinogen, angiotensine I, angiotensine II and saralazin

Biomedical Chromatography : BMC
E BrailoiuG Petrescu

Abstract

Recent studies have described intracellular binding sites for angiotensine II. In vascular smooth muscle it was found that intracellular injection of angiotensin II increases the Ca2+ level. An alternative method for intracellular delivery of drugs is represented by using liposomes. Thus, the aim of this study was to characterize liposomes filled with angiotensinogen, angiotensine I (Ang I), angiotensine II (Ang II), and saralasin by a TLC method and examine the physiological effects of these on rat vascular smooth muscle. Ang II (for all concentrations tested in the aqueous phase) and Ang I (for concentrations less than 10(-4)M) did not affect the thin-layer chromatography migration of this type of vesicle, suggesting that dose-dependent effects on physio-pharmacological experiments could be studied. On the other hand, this type of experiment could not be performed for salarasin- or angiotensinogen-filled liposomes. Administration of liposomes containing Ang II (10(-6)M), Ang I (10(-6)M), angiotensinogen (10(-6)M) and saralasine (10(-6)M) caused the contraction to isolated rat aorta smooth muscle, suggesting the presence of active intracellular binding sites.

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Citations

Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·C M FilipeanuD de Zeeuw
Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·G PetrescuA Costuleanu

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