TLR7 influences germinal center selection in murine SLE

PloS One
Alexis BoneparthAnne Davidson

Abstract

TLR7 enhances germinal center maturation and migration of B cells to the dark zone where proliferation and somatic hypermutation occur. Our goal was to determine how Tlr7 dose influences selection of the autoreactive B cell repertoire in NZW/BXSB. Yaa mice bearing the site-directed heavy chain transgene 3H9 that encodes for the TLR7 regulated anti-CL response. To create a physiologic setting in which autoreactive B cells compete for survival with non-autoreactive B cells, we generated bone marrow chimeras in which disease onset occurred with similar kinetics and the transferred 3H9+ female non-Yaa, male Yaa or male TLR7(-/Yaa) cells could be easily identified by positivity for GFP. Deletion of 3H9 B cells occurred in the bone marrow and the remaining 3H9 follicular B cells manifested a decrease in surface IgM. Although there were differences in the naïve repertoire between the chimeras it was not possible to distinguish a clear pattern of selection against lupus related autoreactivity in TLR7(-/Yaa) or female chimeras. By contrast, preferential expansion of 3H9+ B cells occurred in the germinal centers of male Yaa chimeras. In addition, although all chimeras preferentially selected 3H9/Vκ5 encoded B cells into the germinal cent...Continue Reading

References

Jan 1, 1994·Annual Review of Immunology·M Z Radic, M Weigert
Apr 1, 1993·The Journal of Experimental Medicine·M Z RadicM Weigert
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaobo WangAnne Davidson
Dec 18, 2002·The Journal of Experimental Medicine·Yijin LiMartin Weigert
Aug 16, 2003·Science·Hedda WardemannMichel C Nussenzweig
Jan 22, 2004·International Immunology·Elahna PaulMichael C Carroll
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meera RamanujamAnne Davidson
Feb 18, 2006·The Journal of Clinical Investigation·Meera RamanujamAnne Davidson
May 20, 2006·Science·Prapaporn PisitkunSilvia Bolland
Jun 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Srividya SubramanianEdward K Wakeland
Sep 18, 2007·Advances in Immunology·Hedda Wardemann, Michel C Nussenzweig
Jan 9, 2008·The Journal of Experimental Medicine·Almut Meyer-BahlburgDavid J Rawlings
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lena SchifferAnne Davidson
Jun 4, 2008·European Journal of Immunology·Anna-Marie FairhurstEdward K Wakeland
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie-Laure Santiago-RaberShozo Izui
Sep 2, 2008·Arthritis and Rheumatism·Philip KahnAnne Davidson
Dec 17, 2008·Annals of the New York Academy of Sciences·Taro Kawai, Shizuo Akira
Oct 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Raja VuyyuruZiaur S M Rahman
Oct 19, 2010·Arthritis and Rheumatism·Zheng LiuAnne Davidson
Nov 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Weiqing HuangAnne Davidson
Jul 27, 2012·Current Opinion in Rheumatology·Keith B Elkon, Alice Wiedeman
Sep 5, 2012·Immunologic Research·Anatoly V RubtsovPhilippa Marrack
Oct 2, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ioana MoisiniAnne Davidson
Nov 15, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sun-Hee HwangAnna-Marie Fairhurst
Apr 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yi Ting KohDwight H Kono
Jun 14, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan M Clingan, Mehrdad Matloubian
Jan 1, 2014·European Journal of Immunology·Greg A KirchenbaumLawrence J Wysocki
Jan 1, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhaolin HuaAnthony L DeFranco
Apr 9, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shaun W JacksonDavid J Rawlings
May 9, 2014·Nature·Alexander D GitlinMichel C Nussenzweig
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Zahra SabouriChristopher C Goodnow
Sep 26, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chetna SoniZiaur S M Rahman

❮ Previous
Next ❯

Citations

Jun 13, 2015·International Journal of Molecular Sciences·Kongyang MaLiwei Lu
Dec 20, 2015·Immunological Reviews·Philipp Yu
Sep 1, 2015·Frontiers in Immunology·Megan WoodsAnne Davidson
Jan 28, 2018·Science Immunology·Mélanie SouyrisJean-Charles Guéry
Jun 1, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Hong-Qiong QinZhen-You Jiang
Dec 20, 2020·Nature Reviews. Rheumatology·Simon FillatreauThomas Dörner

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.