TLR9 ligand induces the generation of CD20+ plasmablasts and plasma cells from CD27+ memory B-cells.

Frontiers in Immunology
Alexandrine Geffroy-LuseauCatherine Pellat-Deceunynck

Abstract

Plasma cells (PCs) have a heterogeneous phenotype in humans. While bone marrow PCs are CD20-CD138+, tonsil PCs are CD20+CD138+/- and peripheral plasmablasts (PBs) are CD20-CD138-. In vitro, PCs are mainly generated by the activation of CD27+ memory B-cells through transient stimulation of CD40, and their phenotype appears similar to that of bone marrow PCs. While CD20 expression is lost at the plasmablastic stage, CD138 expression appears only at the PC stage. Thus, the CD20+CD138± phenotype of tonsil PCs does not represent an intermediate stage in the differentiation of memory B-cells into PCs. Because it has been previously shown that TLR9 activation was more able than CD40 stimulation to induce the differentiation of IgM+ CD27+ B-cells, we wondered whether TLR9 or CD40 stimulation would induce the same phenotype of PCs. Thus, we compared the differentiation of CD27+ B-cells isolated from either the tonsils or peripheral blood and stimulated with either CD40L-expressing fibroblasts or a TLR9 ligand, CpG oligodeoxynucleotide (CpG ODN). We observed that CpG ODN mainly induced CD27+ B-cell differentiation into CD20+CD38+CD138- PBs and CD20+CD38+CD138± PCs, which appear similar to tonsil PCs. Removal of CpG ODN during differentia...Continue Reading

Citations

Dec 30, 2014·Cellular and Molecular Life Sciences : CMLS·Mariann KremlitzkaAnna Erdei
Nov 9, 2016·Best Practice & Research. Clinical Haematology·Ramón García-SanzMartín Pérez-Andrés
Feb 11, 2018·Journal of Gastroenterology and Hepatology·Hiroyoshi DoiHitoshi Yoshida
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Apr 20, 2018·Frontiers in Immunology·Walaa DarwicheHussein Ghamlouch
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Methods Mentioned

BETA
ELISA
flow cytometry

Software Mentioned

Modfit

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