TMEM166 inhibits cell proliferation, migration and invasion in hepatocellular carcinoma via upregulating TP53.

Molecular and Cellular Biochemistry
Jiejie YangNing Li

Abstract

Transmembrane protein 166 (TMEM166), an endoplasmic reticulum-associated protein, functions in many diseases via regulating autophagy and/or apoptosis. However, the role of TMEM166 in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we detected the expression of TMEM166 in HCC by real-time fluorescent quantitative PCR (RT-qPCR), immunohistochemistry and western blot. To investigate its biological function and underlying mechanism in HCC, TMEM166 was overexpressed in HCC cell lines and assessed its effects on cell proliferation, migration, invasion, apoptosis and cell cycle by MTT assay, wound healing assay, Transwell assay, Annexin V-FITC/PI assay, JC-1 staining and flow cytometry assay, respectively. Results demonstrated that the expression of TMEM166 was significantly decreased in HCC and was associated with advanced TNM clinical stage and poor clinical outcome of HCC patients. TMEM166 overexpression inhibited HCC cells proliferation, migration and invasion. Furthermore, TMEM166 inhibited cell proliferation by inducing apoptosis and cell cycle arrest via upregulating anti-oncogene TP53 and TP53 knockdown significantly alleviated the anti-tumor effects of TMEM166 on HCC cells. This study provides the firs...Continue Reading

References

Feb 24, 1995·Cell·E M MiyashitaD A Thorley-Lawson
Jul 21, 2001·Molecular Cell·K Nakano, K H Vousden
Oct 8, 2005·Science·Diana SpieringsDouglas R Green
May 12, 2007·Apoptosis : an International Journal on Programmed Cell Death·Lan WangYingyu Chen
May 13, 2008·Gastroenterology·Hashem B El-SeragK Rajender Reddy
Apr 13, 2012·Applied Immunohistochemistry & Molecular Morphology : AIMM·Dong XuYing Yu Chen
Oct 27, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Wei SunJian-Bing Ding
Feb 18, 2014·Gut·Jordi BruixVincenzo Mazzaferro
Sep 23, 2014·International Journal of Cancer. Journal International Du Cancer·Hongmei ZengXue Qin Yu
Nov 5, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Guo-Dong LuShing Chuan Hooi
Feb 3, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Ming TaoYing-Yu Chen
Feb 26, 2016·Stem Cell Reports·Mengtao LiYun Bai
Feb 12, 2017·Experimental Cell Research·Xue ShenYun Bai
Jun 1, 2017·International Journal of Oncology·Xiaofei ZhangGong Yang
Nov 18, 2017·Cell Death and Differentiation·Brandon J AubreyAndreas Strasser
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jul 25, 2018·Alimentary Pharmacology & Therapeutics·Matthias Pinter, Markus Peck-Radosavljevic
Sep 8, 2018·Cancer Science·Satomi Yogosawa, Kiyotsugu Yoshida
Jan 1, 2018·Cell Death and Differentiation·Brandon J AubreyAndreas Strasser

❮ Previous
Next ❯

Citations

Jan 4, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yedda LiMark S Sands

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
surgical resection
FCS
fluorescence microscopy
flow cytometry
transfection
fluorescence

Software Mentioned

GraphPad Prism
Image J

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis