TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation

PloS One
Aurélie HayezOlivier De Backer

Abstract

TMEM45A gene encodes an initially uncharacterized predicted transmembrane protein. We previously showed that this gene is highly expressed in keratinocytes where its expression correlates with keratinization, suggesting a role in normal epidermal physiology. To test this hypothesis, we generated TMEM45A knockout mice and found that these mice develop without any evident phenotype. The morphology of the epidermis assessed by histology and by labelling differentiation markers in immunofluorescence was not altered. Toluidine blue permeability assay showed that the epidermal barrier develops normally during embryonic development. We also showed that depletion of TMEM45A in human keratinocytes does not alter their potential to form in vitro 3D-reconstructed epidermis. Indeed, epidermis with normal morphogenesis were generated from TMEM45A-silenced keratinocytes. Their expression of differentiation markers quantified by RT-qPCR and evidenced by immunofluorescence labelling as well as their barrier function estimated by Lucifer yellow permeability were similar to the control epidermis. In summary, TMEM45A gene expression is dispensable for epidermal morphogenesis, keratinization and barrier formation. If this protein plays a role in t...Continue Reading

References

Sep 12, 1998·The Journal of Investigative Dermatology. Symposium Proceedings·K C MadisonP W Wertz
May 7, 1999·Experimental & Molecular Medicine·Z Nemes, P M Steinert
Oct 26, 2001·The Journal of Investigative Dermatology·L Norlén
Feb 20, 2002·The Journal of Investigative Dermatology·Michal JarnikAlasdair C Steven
May 14, 2004·The Journal of Investigative Dermatology·Akemi Ishida-YamamotoHajime Iizuka
May 14, 2004·The Journal of Investigative Dermatology·Manigé Fartasch
Nov 13, 2009·Methods in Molecular Biology·Frédéric MinnerYves Poumay
Nov 21, 2012·Experimental Dermatology·Aurélie FrankartYves Poumay
Jun 25, 2013·Biochimica Et Biophysica Acta·Leopold EckhartWim Declercq
Aug 16, 2013·Nature·Slobodan BeronjaElaine Fuchs
Dec 3, 2013·The Journal of Investigative Dermatology·Kaori InoueSchuichi Koizumi
Feb 6, 2014·Biological Chemistry·Peter Arne GerberPeter Hevezi
Apr 3, 2014·Experimental Dermatology·Aurélie HayezYves Poumay
Apr 16, 2014·The Journal of Investigative Dermatology·Atsunari TsuchisakaTakashi Hashimoto

❮ Previous
Next ❯

Citations

Jan 31, 2018·Experimental Dermatology·Shreya BhattacharyaMaria I Morasso
Dec 6, 2019·Cell Death & Disease·Kathleen SchmitCarine Michiels
May 25, 2021·Differentiation; Research in Biological Diversity·Hanqian ZhangHans Törmä
Aug 20, 2021·JAMA Ophthalmology·Chen WeinerEran Pras

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping
electrophoresis
electron microscopy
transmission electron microscopy
fluorescence microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta morphologica Academiae Scientiarum Hungaricae
L Szodoray, K Nagy-Vezekényi
Archives of Dermatological Research
F W Bauer, J B Boezeman
Archives of Dermatological Research
D Broekaert, P Van Oostveldt
© 2022 Meta ULC. All rights reserved