TMPRSS2 and MSPL Facilitate Trypsin-Independent Porcine Epidemic Diarrhea Virus Replication in Vero Cells

Viruses
Wen ShiYijing Li

Abstract

Type II transmembrane serine proteases (TTSPs) facilitate the spread and replication of viruses such as influenza and human coronaviruses, although it remains unclear whether TTSPs play a role in the progression of animal coronavirus infections, such as that by porcine epidemic diarrhea virus (PEDV). In this study, TTSPs including TMPRSS2, HAT, DESC1, and MSPL were tested for their ability to facilitate PEDV replication in Vero cells. Our results showed that TMPRSS2 and MSPL played significant roles in the stages of cell-cell fusion and virus-cell fusion, whereas HAT and DESC1 exhibited weaker effects. This activation may be involved in the interaction between TTSPs and the PEDV S protein, as the S protein extensively co-localized with TMPRSS2 and MSPL and could be cleaved by co-expression with TMPRSS2 or MSPL. Moreover, the use of Vero cells expressing TMPRSS2 and MSPL facilitated PEDV replication in the absence of exogenous trypsin. In sum, we identified two host proteases, TMPRSS2 and MSPL, which may provide insights and a novel method for enhancing viral titers, expanding virus production, and improving the adaptability of PEDV isolates in vitro.

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
Mar 19, 1977·The Veterinary Record·E N Wood
Nov 1, 1988·Journal of Clinical Microbiology·M Hofmann, R Wyler
Dec 1, 1988·The Journal of General Virology·W SpaanM C Horzinek
Dec 1, 1983·Nihon juigaku zasshi. The Japanese journal of veterinary science·K TakahashiK Ohshima
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y KitamotoJ E Sadler
Mar 1, 1997·American Journal of Respiratory Cell and Molecular Biology·S YasuokaT Sano
Jun 13, 1998·The Journal of Biological Chemistry·K YamaokaS Yasuoka
Aug 24, 2001·Nature·S M DhanasekaranA M Chinnaiyan
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates
Jan 11, 2007·Journal of Microscopy·S Bolte, F P Cordelières
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Oct 31, 2008·Journal of Virology·Miyuki KawaseFumihiro Taguchi
Apr 29, 2009·Journal of Biochemistry·Yuka MiyakeKuniyo Inouye
Jun 3, 2009·The Journal of Biological Chemistry·Thomas H BuggeQingyu Wu
Jul 25, 2009·Emerging Infectious Diseases·Suphasawatt PuranavejaRoongroje Thanawongnuwech
Jul 16, 2010·Journal of Virology·Stephanie BertramStefan Pöhlmann
May 27, 2011·Journal of Virology·Kazuya ShiratoFumihiro Taguchi
Jul 5, 2011·Annual Review of Cell and Developmental Biology·Roman Szabo, Thomas H Bugge
Jan 21, 2012·Emerging Infectious Diseases·Rui-Qin SunChang-Xu Song
Aug 15, 2014·Journal of Virology·Pawel ZmoraStephanie Bertram

❮ Previous
Next ❯

Methods Mentioned

BETA
IECs
electrophoresis
PCR
Two-Hybrid
transfection
fluorescence-activated cell sorting

Software Mentioned

Image J
ABI 7500 system
Just Another Colocalization Plugin
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.