Tn6167, an antibiotic resistance island in an Australian carbapenem-resistant Acinetobacter baumannii GC2, ST92 isolate

The Journal of Antimicrobial Chemotherapy
Steven J Nigro, Ruth M Hall

Abstract

To determine the context and location of the bla(OXA-23) carbapenem-resistance gene and the structure of the resistance island in the chromosomal comM gene in a representative Australian global clone 2 (GC2) Acinetobacter baumannii isolate. Long-range PCR was used to link genes and determine the organization of the resistance island. PCR amplicons were sequenced, and bioinformatic analysis identified features. Multilocus sequence typing (MLST) was performed. The GC2 isolate A91 is sequence type (ST) ST92 (Oxford MLST scheme). It includes a 37 kb genomic resistance island, Tn6167, in the comM gene. At one end, Tn6167 carries Tn6022Δ1 interrupted by a novel insertion sequence, ISAba17. The sul2 (sulphonamide resistance) and strA-strB (streptomycin resistance) genes and tet(B) tetracycline resistance determinant are at the other end in the configuration ISAba1-sul2-CR2Δ-tetA(B)-tetR(B)-CR2-strB-strA with part of the tni end of a Tn6022-related transposon preceding them and an orf4 end following them. Transposon Tn2006 carrying bla(OXA-23) was found in an 11 kb region located between Tn6022Δ1 and the other resistance genes. The 17.6 kb Tn6166 from the GC2 reference strain A320/RUH134 can be derived from Tn6167 via a single deletion...Continue Reading

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Oct 25, 2011·The Journal of Antimicrobial Chemotherapy·Steven J Nigro, Ruth M Hall

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Citations

Nov 14, 2012·Antimicrobial Agents and Chemotherapy·Elisabet VilacobaMaría Soledad Ramírez
Aug 12, 2014·The Journal of Antimicrobial Chemotherapy·Steven J Nigro, Ruth M Hall
Nov 30, 2014·The Journal of Antimicrobial Chemotherapy·Steven J NigroRuth M Hall
Jan 13, 2016·The Journal of Antimicrobial Chemotherapy·Steven J Nigro, Ruth M Hall
Sep 30, 2015·The Journal of Antimicrobial Chemotherapy·Mohammad HamidianRuth M Hall
Aug 6, 2014·Molecular Microbiology·Joseph E PetersMichael T Petassi
Nov 7, 2012·International Journal of Antimicrobial Agents·Raffaele ZarrilliAthanassios Tsakris
Jul 11, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Eduardo Carneiro ClímacoAna Lúcia da Costa Darini
Jan 12, 2017·The Journal of Antimicrobial Chemotherapy·Grace A BlackwellRuth M Hall
Jun 24, 2015·Antimicrobial Agents and Chemotherapy·Dae Hun Kim, Kwan Soo Ko
Dec 17, 2015·Antimicrobial Agents and Chemotherapy·Grace A BlackwellRuth M Hall
Nov 20, 2018·The Journal of Antimicrobial Chemotherapy·Steven J NigroRuth M Hall
Oct 11, 2019·Microbial Genomics·Mohammad Hamidian, Steven J Nigro
Jul 2, 2017·The Journal of Antimicrobial Chemotherapy·Mohammad Hamidian, Ruth M Hall

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