Tn9 and IS1 inserts in a ribosomal ribonucleic acid operon of Escherichia coli are incompletely polar.

Journal of Bacteriology
J M Brewster, E A Morgan

Abstract

Transcription is known to be coupled to translation in many or all bacterial operons which code for proteins. In these operons, nonsense codons which prevent normal translation often result in premature termination of transcription (polarity). However, efficient transcription of ribosomal ribonucleic acid operons (rrn operons) occurs, although rrn transcripts are not translated. It therefore seemed possible that insertion sequences and transposable elements which are polar in protein-coding operons might not be polar in rrn operons. Previously, it has been shown (E. A. Morgan, Cell 21:257-265, 1980) that Tn10 is incompletely polar in the rrnX operon. Here we show that the transposon Tn9 and the insertion sequence IS1 also incompletely polar in rrnX. In normal cells expression of sequences distal to the insertions can be detected by genetic methods. In ultraviolet-irradiated cells expression of distal sequences is about 80% of that observed in uninterrupted rrnX operons. These observations provide evidence that ribonucleic acid polymerase molecules beginning at rrnX promoters can read through Tn9 and IS1 and that, at least in ultraviolet-irradiated cells, read-through is very efficient.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Ohtsubo, E Ohtsubo
Jan 1, 1977·Annual Review of Genetics·M Nomura, E A Morgan
Jan 1, 1978·Annual Review of Biochemistry·S Adhya, M Gottesman
Apr 1, 1979·Journal of Bacteriology·T J PollockI Tessman
Jan 1, 1980·Journal of Bacteriology·T CabezónA Bollen
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H M JohnstonJ R Roth
Dec 1, 1980·Journal of Bacteriology·J S Fassler, I Tessman

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Citations

Oct 18, 2003·Journal of Bacteriology·Hyuk Kyu SeohCatherine L Squires
Dec 2, 2009·Journal of Bacteriology·Michael YoungCharles L Greenblatt
Dec 1, 1995·Microbiological Reviews·C CondonC L Squires
Oct 1, 1986·Journal of Bacteriology·E A Morgan
Feb 1, 1987·Journal of Bacteriology·G J Murakawa, D P Nierlich
Jul 1, 1984·Journal of Bacteriology·S AksoyC Squires
Oct 5, 1986·Journal of Molecular Biology·P PrentkiD J Galas

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