TNF-α induces vascular endothelial cells apoptosis through overexpressing pregnancy induced noncoding RNA in Kawasaki disease model

The International Journal of Biochemistry & Cell Biology
Chunming JiangXianmei Huang

Abstract

Kawasaki disease (KD) is an autoimmune disease in which the medium-sized blood vessels throughout the body become inflamed. The increased evidences showed that TNF-α was association with vascular inflammation in KD patients. However the detailed mechanism was still unclear. Recent studies indicated abnormal expressed long non-coding RNAs (LncRNAs) involved in many diseases. Thus the purpose of this study is to explore the role of lncRNAs in KD and find out the new target for KD treatment. In this study, firstly we verified the overexpressed TNF-α in KD patients, and found TNF-α was able to induce HUVECs apoptosis and inhibit HUVECs proliferation. After this we screened out pregnancy induced noncoding RNA (PINC) was significantly overexpression in TNF-α treated HUVECs. We also found PINC overexpressed in KD patients. For further study, we designed two siRNA of PINC. After silenced the expression of PINC in HUVECs, we found the Knockdown of PINC enhanced the viability of HUVECs treated with TNF-α, and increased the expression of anti-apoptotic and reduced the expression of apoptotic gene. These results suggest PINC involves in the process of TNF-α induces vascular endothelial cell apoptosis, it may become a new target for KD trea...Continue Reading

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Citations

Mar 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Minoru TanakaHiromi Rakugi
Nov 2, 2019·International Journal of Molecular Sciences·Li ZhangFeng Wang
Jan 31, 2020·Oxidative Medicine and Cellular Longevity·Qidong CaoChunli Song
May 13, 2018·Non-coding RNA·Gabriel A CipollaDaniela F Gradia
Feb 26, 2021·Clinical and Experimental Medicine·Qian WuJun Yang
Mar 3, 2021·European Journal of Pharmacology·Xuan LiHaitao Lv
Jun 30, 2021·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Yan DongXietong Wang
Jul 13, 2021·Frontiers in Pediatrics·Kaushal SharmaSurjit Singh

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