TNF-α promotes colon cancer cell migration and invasion by upregulating TROP-2

Oncology Letters
Peng Zhao, Zhongtao Zhang

Abstract

High levels of tumor-associated calcium signal transduction protein (TROP)-2 have been demonstrated to be strongly associated with tumor necrosis factor (TNF)-α levels in colon cancer. In the present study, the effect of TNF-α on the regulation of TROP-2 expression and its effect in colon cancer cell migration and invasion were investigatedin vitro. TROP-2 protein levels were evaluated in HCT-116 human colon cancer cells cultured with various concentrations of TNF-α using western blot analysis. Changes in the migratory and invasive potential of the cells were evaluated using a wound healing and transwell assay, respectively. Then, TROP-2 expression was downregulated in cells by use of siRNA, and TROP-2 knockdown was confirmed at the mRNA and protein level by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The migration and invasion potential of cells transfected with TROP-2 siRNA was also evaluated. Levels of several mitogen-activated protein kinase proteins, namely p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK), were detected in cells treated with TNF-α using western blot analysis. The results demonstrated that TROP-2 protein levels increased ...Continue Reading

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Citations

May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anjugam ParamananthamWon Sup Lee
Nov 15, 2020·Cancers·Sára LenártPetr Beneš
Jul 27, 2021·Frontiers in Oncology·Apriliana E R KartikasariMagdalena Plebanski
Jul 25, 2021·International Journal of Molecular Sciences·Takamichi ItoYumiko Kaku-Ito
Sep 18, 2021·Journal of Cancer Research and Clinical Oncology·Shota OmoriToshiaki Takahashi

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