PMID: 8958446Sep 1, 1996Paper

TNF-alpha enhances Toxoplasma gondii cyst formation in human fibroblasts through the sphingomyelinase pathway

Cellular Signalling
J RicardP Ambroise-Thomas

Abstract

The cystogenesis event of Toxoplasma gondii is poorly understood. In order to throw light on it, the effect of tumor necrosis factor-alpha (TNF-alpha) was studied in the Prugniaud strain of the organism. This showed that TNF-alpha increased the number of cysts formed in vitro in human MRC5 fibroblasts. The sphingomyelinase pathway may be involved in mediating the TNF effect, since ceramide (natural form in permeabilized cells or cell-permeable analogue) could mimic the action of TNF. More precisely, our results strongly suggest the involvement of an acidic sphingomyelinase in mediating the effect of TNF; indeed, D609 inhibited both the TNF effect and cyst formation, while arachidonic acid had no effect. Moreover, protein kinase (PKC) seems also to play a role in the process, since phorbol-12-myristate-13-acetate (PMA) enhanced the cyst formation. However, chelerythrine chloride did not prevent the TNF effect, suggesting that several host-cell pathways can affect the cystogenesis event. Taken together, these results suggest the active participation of host-cell components in the cystogenesis of Toxoplasma gondii.

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Citations

Jun 14, 2001·International Journal for Parasitology·D Derouich-GuergourH Pelloux
Jun 14, 2002·International Journal for Parasitology·Nahid AzzouzRalph T Schwarz
Nov 24, 2006·Journal of Neurosurgery. Spine·Takashi KusakabeShoichi Kokubun
May 21, 2010·Trends in Parasitology·Françoise Debierre-Grockiego
May 19, 2004·Trends in Parasitology·John EllisDavid Morrison
Jan 27, 2006·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Masako TokunagaTetsuro Sato
Jun 20, 2003·The Journal of Biological Chemistry·Françoise Debierre-GrockiegoRalph T Schwarz
Nov 7, 2015·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Gungor Cagdas Dincel, Hasan Tarik Atmaca

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