TNF-alpha stimulates the biosynthesis of complement C3 and factor B by human umbilical cord vein endothelial cells

Cancer Letters
Y KawakamiA Ueki

Abstract

The human umbilical cord vein (HUVEC) endothelial cells synthesize and secrete complement components. We analyzed the regulation of biosynthesis of the third component of complement (C3) and factor B (Bf) by cytokines in endothelial cells. Production of C3 increased after stimulation with TNF-alpha or IL-1beta and that of Bf with TNF-alpha, TNF-beta and IFN-gamma. TNF-alpha was the most effective in stimulating the secretion of C3 and Bf or the expression of mRNA of C3 or Bf. Preincubation with TNF-alpha for at least 36 or 24 h was needed for the stimulation, respectively. TNF-alpha, together with IFN-gamma had synergistic effects and the release of C3 or Bf increased to about 40- and 26-fold higher than those in control cells after incubation with both cytokines.

References

Jun 1, 1991·Scandinavian Journal of Immunology·E Johnson, G Hetland
May 1, 1989·The Journal of Experimental Medicine·S K LoS D Wright
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M H de Bruijn, G H Fey
Nov 1, 1988·Arthritis and Rheumatism·Y Katz, R C Strunk
Dec 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M GuiguetG Mack
Mar 1, 1981·Clinical Immunology and Immunopathology·D W BeattyH R Colten

❮ Previous
Next ❯

Citations

Dec 21, 2002·Molecular Immunology·Mauro BongrazioAndreas Zakrzewicz
Oct 7, 2004·Biological & Pharmaceutical Bulletin·Eric Brian HoieTerrence Leo Zach
Dec 17, 2017·Scientific Reports·Eun Jee ChungDemetrios G Vavvas
Jan 31, 2018·Scientific Reports·Martin J PageEtheresia Pretorius
Jul 1, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H RaedlerP S Heeger
Oct 27, 2016·Immunological Reviews·Lubka T RoumeninaVeronique Fremeaux-Bacchi
Dec 22, 1999·The American Journal of Physiology·E J TanhehcoB R Lucchesi
Dec 18, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah E SartainJoel L Moake
May 18, 2021·American Journal of Physiology. Renal Physiology·Sorena LoNirmala Parajuli
Jan 30, 2007·Prostaglandins & Other Lipid Mediators·E M ZardiA Afeltra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.