TNFα and TGFβ-1 synergistically increase the cancer stem cell properties of MiaPaCa-2 cells

Oncology Letters
Aikyn KaliNikolai N Belyaev

Abstract

Increased serum concentrations of tumor necrosis factor α (TNFα) and transforming growth factor β-1 (TGFβ-1) in the blood of patients with pancreatic cancer (PC) have previously been demonstrated. In addition, exogenous exposure to these cytokines promotes various cancer cell invasive and cancer stem cell (CSC) phenotypes. However, their importance in pancreatic CSCs remains elusive. In the present study, the effects of TNFα and TGFβ-1 on the human PC cell line MiaPaCa-2 were examined. Using flow cytometry, it was revealed that TNFα and TGFβ-1 synergistically increase cluster of differentiation (CD) 44v6, CD133 and ATP-binding cassette transporter G2 (ABCG2) expressing populations in adherent tumor cell culture conditions. Furthermore, a similar trend was observed in cells pretreated with these cytokines grown in sphere forming culture conditions. Similar to previous studies, TNFα treatment increased the proportion of epidermal growth factor receptor (EGFR) expressing cells in adherent culture, and this data was further supported by the results of the sphere formation assay, in which the subculture with a high proportion of EGFR expressing cells exhibited the most efficient sphere forming ability. However, the proportion of vas...Continue Reading

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Citations

Sep 1, 2020·Immunological Investigations·Yekaterina O OstapchukNikolai N Belyaev
Feb 16, 2019·International Journal of Molecular Sciences·Andrea PadoanDaniela Basso
Jan 12, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mai Abdel Mouti, Siim Pauklin

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

CellQuest Pro

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