TNF but not IL-1 decreases pancreatic acinar cell survival without affecting exocrine function: a study in the perfused human pancreas

The Journal of Surgical Research
W DenhamJ Norman

Abstract

Substantial quantities of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) are produced within the pancreatic parenchyma during acute pancreatitis. Recent evidence suggests that IL-1 beta and TNF-alpha propagate acute pancreatitis and intensify the resulting pancreatic acinar cell death. This study examines the direct effect of IL-1 beta and TNF-alpha on pancreatic acinar cells. Human pancreata (n = 6), harvested during organ procurement, were perfused ex vivo through the splenic artery using a sterile, oxygenated colloid solution. Each pancreas was perfused with either recombinant human IL-1 beta or TNF-alpha for 2 h and subsequently with the cholecystokinin analogue caerulein (positive control). Venous effluent was collected continuously and amylase and lipase were determined at 15-min intervals. Pancreatic histology was graded at baseline and following cytokine and caerulein perfusion. To examine the long-term effects of these cytokines on acinar cell viability, additional in vitro studies utilized the AR42J acinar cell line which was exposed to either IL-1 beta or TNF-alpha with survival determined daily by MTT assay. Perfusion of the human pancreas with either IL-1 beta or TNF-alpha did not alter ...Continue Reading

References

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Citations

Jan 19, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S AggarwalA L Gurney
Nov 15, 2008·World Journal of Gastroenterology : WJG·Georgios I Papachristou
Nov 26, 2010·World Journal of Gastroenterology : WJG·Marcelo-G BinkerLaura-I Cosen-Binker
Nov 20, 2004·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Anna S Gukovskaya, Stephen J Pandol
Jun 11, 2016·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Aparna JakkampudiRupjyoti Talukdar
Oct 4, 2014·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Chathur AcharyaVijay P Singh
Jan 21, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anne Barbara TietzClaus Schäfer
Jul 23, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tae-Hyeon KimSung-Joo Park
Aug 29, 2013·International Journal of Genomics·Elena A ShubinaOleg F Gritsenko
Jul 17, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·B RauH G Beger

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