PMID: 15240725Jul 9, 2004Paper

TNF-induced beta2 integrin activation involves Src kinases and a redox-regulated activation of p38 MAPK

The Journal of Immunology : Official Journal of the American Association of Immunologists
Mohamed BouaouinaP Rieu

Abstract

We previously demonstrated that the TNF-alpha-induced inside-out signaling leading to beta(2) integrin activation is redox regulated. To identify kinases involved in this pathway, the effects of kinase inhibitors on the expression of beta(2) integrin activation neoepitope (clone 24) were investigated. We show that both p38 MAPK (inhibited by SB203580) and Src kinases (inhibited by PP2) are involved in beta(2) integrin activation by TNF and oxidants in human neutrophils. Src kinases appeared constitutively active in resting neutrophils and not further activated by TNF or oxidants in nonadherent conditions. However, PP2 blocked both TNF-induced expression of the 24 epitope and cell adhesion promoted by the integrin activating anti-CD18 KIM185 mAb, showing that both the inside-out and the outside-in signaling involve Src kinases. p38 MAPK was activated by TNF and oxidants in nonadherent conditions i.e., with 10 mM EDTA. This activation in EDTA resulted in CD11b, CD35 and CD66 up-regulation and in an oxidative response, all blocked by SB203580 and PP2. p38 MAPK was not activated upon direct integrin activation by KIM185 mAb. Thus, p38 activation allows the study to distinguish the initial transduction pathway leading to beta(2) int...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J RichterT Andersson
Oct 1, 1995·Immunology Today·M LubC G Figdor
Jan 1, 1996·International Journal of Clinical & Laboratory Research·G BertonC A Lowell
Aug 15, 1998·Annals of the New York Academy of Sciences·N Borregaard
Nov 11, 1999·European Journal of Immunology·E BlouinP Rieu
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A NickG S Worthen
Sep 9, 2000·Current Opinion in Cell Biology·Y van Kooyk, C G Figdor
Nov 21, 2000·Trends in Biochemical Sciences·F Rusnak, T Reiter
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·C LuT A Springer
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C LuT A Springer
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D C CaraP Kubes
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tetsuji KamataYoshikazu Takada
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Judith BrangerTom van der Poll
Apr 24, 2002·Immunity·Attila MócsaiClifford A Lowell
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mimi TakamiLilli Petruzzelli
Sep 18, 2002·Immunological Reviews·M Amin Arnaout
Nov 26, 2002·Journal of Immunological Methods·Caroline GilbertPaul H Naccache
Apr 26, 2003·Blood·Jian-Ping XiongM Amin Arnaout
Jul 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shalini Pereira, Clifford Lowell
Aug 2, 2003·Nature Cell Biology·David A Calderwood, Mark H Ginsberg
Oct 2, 2003·Current Opinion in Cell Biology·Christopher V Carman, Timothy A Springer

❮ Previous
Next ❯

Citations

Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek StrassheimEdward Abraham
Aug 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roland Csépányi-KömiErzsébet Ligeti
Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Irina MiraldaKenneth R McLeish
Nov 21, 2018·The Journal of Clinical Investigation·Deepika SharmaThirumala-Devi Kanneganti
Dec 2, 2006·Journal of Leukocyte Biology·Laurie E KilpatrickHelen M Korchak
Nov 30, 2019·Frontiers in Cellular and Infection Microbiology·Jordan LeroySamir Jawhara
Aug 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Srijana PokhrelPamela R Hall
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alessandra PolenghiMarina de Bernard
Jul 20, 2007·Journal of the American Society of Nephrology : JASN·Soumeya BrachemiLise Halbwachs-Mecarelli
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Khalid SendideZakaria Hmama
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephan PhilippDieter Adam
Jul 29, 2016·Scientific Reports·Qian LiChristine Selhuber-Unkel
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hui S LeeJihee L Kang
Jun 12, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Hélène VancraeynesteSamir Jawhara
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tieming Zhao, Gary M Bokoch
Jun 6, 2009·Journal of Cell Science·Sam J MathewMaria Leptin
Oct 15, 2005·Biochemical and Biophysical Research Communications·Hiroyuki HattoriYasuhito Nakagawa
Sep 25, 2008·Biochemical and Biophysical Research Communications·Karina Alves ToledoLise Halbwachs-Mecarelli
Jul 28, 2009·Mechanisms of Ageing and Development·Thorsten PetersKarin Scharffetter-Kochanek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.