To Explore the Protective Mechanism of PTEN-Induced Kinase 1 (PINK1)/Parkin Mitophagy-Mediated Extract of Periplaneta Americana on Lipopolysaccharide-Induced Cardiomyocyte Injury

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Jie LiLiqun Ren

Abstract

BACKGROUND Sepsis is defined as a systemic inflammatory response syndrome caused by an infection (suspicious or confirmed). Its essence is inflammatory mediators and cytokines mediated by host immune response. The present study aimed to investigate the role of Periplaneta americana extracts (XML) on PTEN-induced kinase 1 (PINK1)/Parkin mediated mitophagy in cardiomyocyte injury by sepsis. MATERIAL AND METHODS H9C2 cells were cultured and transfected with Mdivi-1 and Atg7 siRNA. The cell viability and drug toxicity were detected using Cell Counting Kit-8 assay. ELISA (enzyme-linked immunosorbent assay) was used to assess cardiac injury factors and inflammatory factors. Fluorescence levels of LC3 were detected using immunofluorescence assay. Then, the protein and mRNA expression levels were analyzed using western blot and qRT-PCR. Intracellular adenosine triphosphate (ATP) levels were measured using an ATP kit. Finally, flow cytometry was used to detected apoptosis. RESULTS The result showed that XML significantly increase cell viability in H9C2 cells. Compared with XML+LPS (lipopolysaccharide) group, the level of cTNI, CK-MB, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was significantly upregulation in LPS+XML+M...Continue Reading

Citations

May 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Shan-Shan LinJing-Yuan Mao
Aug 28, 2020·Frontiers in Pharmacology·Yingying ZouXiaowen Zhang
Apr 21, 2020·Oxidative Medicine and Cellular Longevity·Farzaneh ShakeriAmirhossein Sahebkar
Apr 8, 2020·Aging and Disease·Yibo YangBin Liu
Oct 9, 2021·Current Pharmaceutical Design·Li-Ping YuXing-Xin Yang

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Methods Mentioned

BETA
protein assay
transfection
ELISA

Software Mentioned

SPSS

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