To go or not to go: Migration of human mesenchymal progenitor cells stimulated by isoforms of PDGF

Journal of Cellular Biochemistry
Jörg FiedlerR E Brenner

Abstract

The recruitment of mesenchymal progenitor cells (MPCs) and their subsequent differentiation to osteoblasts is mandatory for bone development, remodeling, and repair. To study the possible involvement of platelet-derived growth factor (PDGF) isoforms, primary human MPCs and osteogenic differentiated progenitor cells (dOB) were examined for chemotaxic response to homodimeric human platelet-derived growth factor AA, -BB, and heterodimeric PDGF-AB. The role of PDGF receptors was addressed by preincubation with PDGF receptor alpha and beta chain specific antibodies. Migration of MPCs, dOB, and primary osteoblasts (OB) was stimulated by the addition of rhPDGF-AA, rhPDGF-BB, and rhPDGF-AB. The effect was highest in MPCs and for rhPDGF-BB, and declining with osteogenic differentiation. Preincubation with the receptor alpha specific antibody decreased the CI to borderline values while pretreatment with the receptor beta specific antibody led to a complete loss of chemotactic response to PDGF isoforms. In control experiments, basal migration values and rhBMP-2 as well as rxBMP-4 induced chemotaxis of MPC were not influenced by the addition of receptor alpha or beta antibodies. Interestingly, without preincubation the parallel exposure of...Continue Reading

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Jan 1, 1980·Journal of Immunological Methods·W FalkE J Leonard
Feb 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H E YoungP A Lucas
Aug 1, 1996·Acta Orthopaedica Scandinavica·M Lind
Apr 29, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S P BruderF P Barry
Aug 11, 1998·International Journal of Oral and Maxillofacial Surgery·N R KüblerW Sebald
Apr 2, 1999·Science·M F PittengerD R Marshak
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J E Aubin
Oct 3, 1999·Physiological Reviews·C H Heldin, B Westermark
Sep 16, 2000·Experimental Hematology·R J Deans, A B Moseley
Mar 14, 2001·The Journal of Bone and Joint Surgery. British Volume·U Mayr-WohlfartK P Günther
Sep 27, 2001·Journal of Cellular Physiology·S GronthosJ M Gimble
Oct 25, 2002·Journal of Cellular Biochemistry·Jörg FiedlerRolf E Brenner
Jan 1, 1998·Journal of Cellular Biochemistry·Jane E Aubin

❮ Previous
Next ❯

Citations

Jul 5, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Wolfgang BöckerMatthias Schieker
Jan 8, 2013·Calcified Tissue International·Christa Maes
Mar 3, 2012·Cell and Tissue Research·Hemin NieJeremy J Mao
Sep 10, 2010·Biomechanics and Modeling in Mechanobiology·Véronique PeifferLiesbet Geris
May 30, 2013·Trends in Molecular Medicine·Chunsik LeeXuri Li
May 12, 2006·The New England Journal of Medicine·Ellin BermanNicholas P Sauter
Mar 14, 2007·Stem Cells and Development·Yoshie OzakiYukio Kato
Jun 12, 2010·Stem Cells and Development·Fatima A SalehPaul G Genever
Apr 13, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Jul 8, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Jul 22, 2010·Tissue Engineering. Part B, Reviews·Martha W BetzJohn P Fisher
May 18, 2013·Tissue Engineering. Part B, Reviews·Wendy S Vanden Berg-Foels
Oct 28, 2008·PloS One·Maria Arantzazu Sanchez-FernandezBernard Hoflack
Aug 7, 2007·International Journal of Hematology·Brett HallFrank Marini
Mar 20, 2013·Molecular Medicine·Nina-Emily HengartnerRolf E Brenner
Jan 7, 2011·Biologics : Targets & Therapy·Tariq I Mughal, Andrew Schrieber
Apr 25, 2013·The International Journal of Biochemistry & Cell Biology·Rebecca KulawigMichaela Endres
Jun 11, 2014·The Journal of Oral Implantology·Prasun ShahJames Rutkowski
May 2, 2007·The Journal of Cell Biology·Stephen G BallCay M Kielty
Nov 29, 2012·Connective Tissue Research·Li Yen ChongPo-Chun Chang
May 19, 2011·Toxicology and Applied Pharmacology·Mirja NurmioTiina Laitala-Leinonen
Nov 9, 2010·Foot and Ankle Clinics·Christopher W DiGiovanni, James M Petricek
May 25, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu-Lan HuJian-Qing Gao
Sep 3, 2008·Journal of Theoretical Biology·Pedro MoreoManuel Doblaré
Mar 21, 2008·Journal of Theoretical Biology·Hanna IsakssonKeita Ito
Apr 28, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Elisabetta CenniNicola Baldini
Sep 22, 2009·Journal of Internal Medicine·W Richter
Jan 23, 2009·Veterinary Dermatology·Jung-Hyun KimHee-Myung Park
Apr 16, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ayano TokunagaMasakiyo Sasahara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.